Words similar to centrifugal
Example sentences for: centrifugal
How can you use “centrifugal” in a sentence? Here are some example sentences to help you improve your vocabulary:
cfu = colony forming units; COX-2 = cyclooxygenase-2; DMEM = Dulbecco's modified Eagle's medium; EGFP = enhanced green fluorescent protein; FACS = fluorescence-activated cell sorting; FLS = fibroblast-like synovial cells; MoMLV = Moloney murine leukemia virus; PCR = polymerase chain reaction; RA = rheumatoid arthritis; RCF = relative centrifugal force.
The fractions were concentrated by an Ultrafree-15 centrifugal filter device (Millipore Corporation), and loaded onto an FPLC-connected Superdex 75 HR 10/30 (Pharmacia Biotech) column which had been equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT and 150 mM NaCl.
The mixtures were transferred into a Centricon centrifugal ultrafiltration device with a M w cut-off of 100,000 and centrifuged at 3,500 × g for 30 min.
The viral particles were subjected to three rounds of ultrafiltration with ZM-500 centrifugal filtration units (molecular mass cut-off, 500 kDa; Millipore) using PBS (pH 7.4) containing 0.05% Tween 20 (PBST) as a diluent to remove non-virion-associated biotinylation reagent.
Centrifugal concentration of viral supernatant by 10- to 100-fold significantly increased the efficiency of viral transduction, with 50% or more of FLS expressing EGFP in several independent experiments using FLS lines from separate donors.
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