Words similar to cell-free
Example sentences for: cell-free
How can you use “cell-free” in a sentence? Here are some example sentences to help you improve your vocabulary:
Percent specific release of 51 Chromium was calculated from 40 μl of cell-free supernatant.
Infections were initiated for 30 min, after which cell-free bacteria were rinsed away by washing gx, and then continued in DME + 10% FBS.
Upon termination of the experiments, cell-free supernatants from each culture dish were assayed for TNF-α, MMP-2 and MMP-9, while rat neonatal microglia were processed for RNA isolation and RT-PCR.
First, cell-free extracts of different erythrocytic stages of P. falciparum were subjected to Western blot using a monoclonal antibody that was raised against nearly full-length human PP1 and showed a broad species specificity (see Materials and Methods).
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
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