Words similar to cell-free
Example sentences for: cell-free
How can you use “cell-free” in a sentence? Here are some example sentences to help you improve your vocabulary:
Infections were initiated for 30 min, after which cell-free bacteria were rinsed away by washing gx, and then continued in DME + 10% FBS.
Cell-free membrane-binding assays
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
To test for tight-junction formation, [ 3H]inulin was added to the medium on the apical surface, with cell-free 0.45 μm filters as a control for rapid diffusion.
Upon termination of the experiments, cell-free supernatants from each culture dish were assayed for TNF-α, MMP-2 and MMP-9, while rat neonatal microglia were processed for RNA isolation and RT-PCR.