Words similar to cell-free

Example sentences for: cell-free

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To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
Cell-free virus was harvested, titrated and characterized by PRA and PEA prior to use in subsequent passage experiments (Figure 1).
The tissue was homogenized and virus stocks were prepared in MRC-5 cells from cell-free supernatants.
In order to determine if the increase in TNF-α mRNA after a short term stimulation with either domoic acid [1 mM] or LPS [10 ng/mL] led to a concomitant production of TNF-α protein, the cell-free media corresponding to the same microglia used for TNF-α mRNA analysis were assayed with an ultrasensitive rat-specific ELISA.
Cell-free membrane-binding assays