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Example sentences for: catenation

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Phage PM2 DNA was extracted from infected Pseudoalteromonas espejiana cells [ 43 ] and PM2 DNA with one or more single-chain scissions used in the catenation assay was prepared as described [ 44 ] .
The ZD domain is not required for catenation of double-stranded DNA circles
This catenation activity observed with topoisomerase I and Top67 was unlikely to be due to contaminating topoisomerase III activity since it was not observed with the ZD domain purified under almost identical procedures and a site-directed mutant with substitution of the active site Tyr319 by phenylalanine also did not exhibit this activity (Figure 6a).
E. coli topoisomerase I can catalyze catenation of double-stranded DNA circles if the molecules contain single-strand scissions [ 15 16 ] . To test if the Top67 can carry out double-stranded DNA passage at enzyme cleavage sites across from the DNA nicks, the yield of DNA catenanes were compared with that obtained with topoisomerase I. In contrast to the relaxation activity, the catenating activity of Top67 shown in figure 6was as efficient as that of full-length topoisomerase I, and the addition of the ZD domain had no effect (Figure 6a).
The catenation reaction was carried out with 1.4 μg of nicked PM2 phage DNA in 20 μl of 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 10 mM KCl, 10 mM MgCl 2 . After incubation at 37°C for up to1 h, the reactions were stopped with the addition of 1% SDS and 50 mM EDTA.