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Example sentences for: catenanes

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E. coli topoisomerase I can catalyze catenation of double-stranded DNA circles if the molecules contain single-strand scissions [ 15 16 ] . To test if the Top67 can carry out double-stranded DNA passage at enzyme cleavage sites across from the DNA nicks, the yield of DNA catenanes were compared with that obtained with topoisomerase I. In contrast to the relaxation activity, the catenating activity of Top67 shown in figure 6was as efficient as that of full-length topoisomerase I, and the addition of the ZD domain had no effect (Figure 6a).
High concentration of DNA substrate is required to favor formation of catenanes catalyzed by topoisomerase I, and the enzyme also has to be present in higher concentration compared to the relaxation reaction.
This proposed role for the ZD domain in interacting with the passing single-strand of DNA is also supported by the observation that there is no difference between Top67 and intact topoisomerase I in the formation of catenanes.