Words similar to carboxylate
Example sentences for: carboxylate
How can you use “carboxylate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Efforts to solve the crystal structure of one of the metallo-β-lactamases with a bound substrate molecule have failed, most likely due to the high activity of the enzymes towards all β-lactam containing antibiotics [ 37 54 ] . Therefore, computational studies have been used extensively to study substrate binding, the role of the Zn(II) ions in catalysis, the protonation state of the active site, and inhibitor binding [ 37 42 55 56 57 58 59 ] . All of the substrate binding models have made assumptions before the substrate was docked into the active site [ 37 42 ] , and some of these assumptions have been shown to be invalid for certain substrates [ 43 ] . With L1, two key assumptions were made: (1) the bridging hydroxide functions as the nucleophile during catalysis and (2) Zn 1 coordinates the β-lactam carbonyl [ 37 ] . With these assumptions and after energy minimizations, Ser224 was predicted to hydrogen bond to the substrate carboxylate [ 37 ] , reminiscent of the role predicted for Lys224 in CcrA [ 42 ] . Ullah et al.
An example of the first scenario is o -succinylbenzoate synthase, which catalyzes the dehydration of 2-hydroxy-6-succinyl-2,4-cyclohexadiene carboxylate to form o -succinylbenzoate synthase in the biosynthetic pathway for menaquinone synthesis.
All sequenced subclass Ba and Bb metallo-β-lactamases (except VIM-1) have a lysine residue at position 224 [ 41 ] , and all computational models for substrate binding to the metallo-β-lactamases assume that the invariant carboxylate on substrates forms an electrostatic interaction with this lysine.
These include O -acetylserine sulfhydrylase, threonine deaminase, threonine synthase, cystathionine β-synthase, 1-aminocyclopropane-1-carboxylate deaminase, L-serine dehydratase, and D-serine dehydratase.
The same result was obtained when p66 INSwas dimerized with p51 having Asp→Ala mutation at amino acid position 186 (one of the catalytically crucial carboxylate triad) but having an intact β7-β8 loop.
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