Words similar to cacl

Example sentences for: cacl

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The bath and control perfusion solutions were the same and contained (in mM): 100 NaCl, 2 KCl, 1 MgCl 2 , 1 CaCl 2 , 11 glucose, 10 HEPES at pH 7.4.
Lysate was prepared for heparitinase treatment by adding CaCl 2 to 10 μM final concentration.
Slices were allowed to incubate and recover for at least an hour in room temperature ACSF comprised of the following composition (mM): NaCl 117; KCl 5.4; NaHCO 3 26; MgSO 4 1.3; NaH 2 PO 4 1.2; CaCl 2 2.5; glucose 10, continuously bubbled with 95% O 2 plus 5% CO 2 .
0 dextrose; 1.8 CaCl 2 . The tissues were continuously aerated with a mix of 95% O 2 and 5% CO 2 . The vessel segments used for morphological examination were cleaned of connective tissues and the endothelial cell layer was left intact.
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.