Words similar to cacl
Example sentences for: cacl
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Nucleic acids within permeabilized cores were digested with micrococcal nuclease in the presence of CaCl 2 Micrococcal nuclease was inactivated by addition of EGTA, 1.5 μg RNA was added, and the reaction was performed under conditions described for the endogenous polymerase reaction.
The gels were then incubated for 24 hours at 37°C in 50 mM Tris-HCl buffer, pH 7.4, containing 5 mM CaCl 2 . A duplicate negative control gel was incubated as described above but in 50 mM Tris-HCl, pH 7.4 containing 10 mM EDTA instead of CaCl 2 . The gels were fixed for 1 hour in 40% methanol /7% acetic acid and then stained in Coomassie Blue Solution (Sigma), followed by destaining in 10% methanol, 7% acetic acid.
Mefloquine (2, 10, 30, 60, 80 or 200 μM), thapsigargin (1 μM), glutamate (1 μM) and CaCl 2 (1.
The phalloidin-stabilized α-actin filaments (1 μM) were incubated in buffer F (5 mM Tris-HCl, pH 7.5, 0.5 mM Na 2 ATP, 2 mM MgCl 2 , 140 mM NaCl, 0.2 mM DTT, 0.2 mM CaCl 2 , 0.005% sodium azide) with or without 0.1 % DOTMAC for 10 min at 25°C.
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.
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