Words similar to cacet

Example sentences for: cacet

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For example, a probe specific for the 5' end of the CaCET1 gene (probe A in Figure 1) hybridized to a single 4.4 kbp Bgl II fragment after restriction digestion of total DNA from the parental diploid strain, whereas the heterozygote contained an additional 2.7-kbp fragment derived from scission at a novel Bgl II site located within the ARG4 component of the UAU1 insert of the disrupted cacet1::UAU1 allele (Figure 2A, lane P versus lane H).
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
Test of CaCET1 Essentiality in C. albicans
5-kbp fragment corresponding to cacet1::UAU1 (data not shown).