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Example sentences for: cacet

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To address these issues, we have characterized the RNA triphosphatases of two other fungi, including the human pathogen Candida albicans and the fission yeast Schizosaccharomyces pombe [ 10 11 12 ] . The fungal triphosphatases, S. cerevisiae Cet1, C. albicans CaCet1 and S. pombe Pct1, belong to a new family of metal-dependent phosphohydrolases that embraces the triphosphatase components of DNA virus and protozoan mRNA capping systems [ 1 7 13 14 ] . The defining features of the metal-dependent RNA triphosphatases are two glutamate-containing motifs that are required for catalysis and comprise the metal-binding site in the crystal structure of S. cerevisiae Cet1.
Here we have shown that the RNA triphosphatases Pct1 and CaCet1 are essential for viability of S. pombe and C. albicans, respectively The conclusion that CaCet1 is essential is based on a finding that none of the 54 independent isolates in the single-transformation test were homozygous for cacet1Δ; our interpretation is consistent with criteria established by Mitchell and colleagues for inference of essentiality using this genetic approach.
Test of CaCET1 Essentiality in C. albicans
Mutational analysis of the Candida triphosphatase suggested strongly that the tunnel fold and the constituents of the active site are similar, if not identical, in Cet1 and CaCet1 [ 10 ] .
We found that the heterozygous CaCET1/cacet1::UAU1 strain displayed normal growth and morphology (not shown).