Words similar to cacet

Example sentences for: cacet

How can you use “cacet” in a sentence? Here are some example sentences to help you improve your vocabulary:

We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
CaCET1 gene and an intervening marker cassette ( ura3Δ3'-ARG4-ura3Δ5', referred to as UAU1 ) composed of the C. albicans ARG4 gene flanked by overlapping 5' and 3' fragments of the URA3 gene.
We found that 54/54 Arg +Ura +isolates retained the wild-type CaCET1 locus (Figure 2and data not shown), implying that CaCET1 is an essential gene.
Correct insertion via homologous recombination into one copy of the CaCET1 gene, resulting in cacet1::UAU1 (Figure 1), was confirmed by Southern blotting of genomic DNA digested with diagnostic restriction endonucleases.
The blots were probed with a 5' specific CaCET1 fragment (probe A in Figure 1), which detects both the wild-type CaCET1 allele and the cacet1::UAU1 allele, and with a 3'-specific CaCET1 fragment (probe B in Figure 1) derived from the segment deleted during construction of the cacet1::UAU1 disruption cassette.