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Example sentences for: cacet

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We found that the heterozygous CaCET1/cacet1::UAU1 strain displayed normal growth and morphology (not shown).
Recombination within UAU1 to regenerate URA3 eliminates the Bgl II site and results in a cacet1::URA3 locus that would yield an ~5-kbp fragment upon digestion with Bgl II (Figure 1).
However, if CaCET1 is essential for growth, then all of the Arg +Ura +isolates will have three copies of the CaCET1 locus ( cacet1::UAU1/cacet1:: URA3/CaCET1 ).
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
We found that 54/54 Arg +Ura +isolates retained the wild-type CaCET1 locus (Figure 2and data not shown), implying that CaCET1 is an essential gene.