Example sentences for: buffered

How can you use “buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After incubation of the flasks for 2 h, the cells were washed twice, and then resuspended, in 5 ml phosphate buffered saline.

  • Peptides and cells were prepared in Tris Buffered Saline Binding Buffer: 50 mM Tris, 120 mM NaCl, 0.2 mg/ml bacitracin, 20 μg/ml leupeptin, 20 μg/ml chymostatin, 0.1 % bovine serum albumin, pH 7.4.

  • AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; DMEM, Dulbecco's modified Eagle medium; FBS, fetal bovine serum certified; FCC, ferricytochrome c type III; HBSS, Hank's balanced salt solution; LPS, lipopolysaccharide; MMP-9, matrix metalloproteinase-9; O 2 -, superoxide; P, penicillin; PBS, phosphate buffered saline; PMA, phorbol 12-myristate 13-acetate ; RT-PCR, reverse transcriptase polymerase chain reaction; S, streptomycin; SOD, superoxide dismutase; TNF-α, tumor necrosis factor.

  • Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.

  • Two-milliliter samples of whole blood were collected in buffered sodium citrate, and high molecular weight genomic DNA was obtained from the peripheral blood leukocyte fraction (QIAamp Blood Tissue Kit, Qiagen, Valencia CA).


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