Example sentences for: buffered

How can you use “buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were then washed with phosphate buffered saline (PBS) and then incubated in medium (Ham's F12 or Dulbecco's modified Eagle's medium (DMEM)) containing 100 μM bile acids for 1 to 5 h as detailed in the Figure legends.

  • An area 7 to 8 mm in diameter, which included the uninjured epithelial margins, was removed, bisected in the midtransversal plane, fixed in 10% formalin (Buffered Formalde-Fresh, Fisher Scientific, Fair Lawn, NJ) and embedded in paraffin.

  • Lenses were preserved in a primary fixative of 10% buffered formalin (in 0.1 M phosphate buffer, pH 7.2) for 24 hours at room temperature with slight agitation [ 12 ] . This was followed by five days of fixation in an osmotically balanced solution of 2.5% gluteraldehyde in 0.12 M sodium cacodylate buffer (pH 7.2) at room temperature with daily fix exchanges.

  • After washing in Tris buffered saline containing 0.1% Tween 20 (TBST, pH 7.4), blots were incubated for 30 minutes at room temperature with anti-IgG secondary antibody conjugated to horseradish peroxidase.

  • The pellets were dissolved in lysis buffer [1% Nonidet P40 {NP40}, 100 μg/ml bovine serum albumin (BSA) in phosphate buffered saline (PBS)], incubated for 30 min at 20°C and then added to wells coated with rabbit polyclonal antibodies to the gp41 core.


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