Example sentences for: buffered

How can you use “buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Plates were washed three times in phosphate buffered saline (PBS) (pH 7.4) and blocked with 5% bovine serum albumin (BSA) in PBS before serum was added.

  • Tissues were fixed in 10% buffered formalin and paraffin-embedded.

  • Cells were then washed with Hank's buffered sterile saline solution and homogenized in 2 ml Triazol Reagent (Gibco/BRL), and RNA was purified in accordance with the manufacturer's instructions.

  • Embryos were dissected in cold phosphate buffered saline (PBS) and staged according to Theiler [ 68 ] . SMGs were dissected and cultured, processed for histology, or stored at -70°C.

  • For transfections, 10 5HepG2 cells were plated in 24 well dishes with DMEM supplemented with 10% charcoal stripped serum and transfected using the calcium phosphate precipitation method as described [ 16 ] . The next morning, cells were washed with phosphate buffered saline and ligands were added.


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