Words similar to buffered

Example sentences for: buffered

How can you use “buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:

For coating with antibodies, wells were first coated with protein A (1 μg/well) in 0.1 M Tris buffer, pH 8.8 for 2 h at 20°C followed by either mAbs (1 μg/well) or rabbit antisera diluted 1:100 in phosphate buffered saline (PBS) for 1 h at 20°C.
9% saline, 10% Phosphate Buffered Formalin).
Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Dulbecco's phosphate buffered saline (DPBS), antibiotic/antimycotic, 1% trypsin/EDTA, Hanks Balanced Salt Solution (HBSS) and TRIZOL were purchased from Invitrogen Gibco Cell Culture (Carlsbad, CA).
Collagen type I coated plates were prepared by incubating tissue culture plates with 0.1 mg rat tail collagen per ml Phosphate Buffered Saline (PBS) for 3 hrs at room temperature, followed by a 30 min, 37°C incubation with 0.5%BSA (bovine serum albumin) in PBS with 0.5 mM Ethylene DiamineTetraacetic acid (EDTA) - PBS/EDTA, and final PBS washes.
Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.