Words similar to buffer-saline

Example sentences for: buffer-saline

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Sections were incubated with 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Triton X-100; 30 min) followed by incubation with MIF antibody (rabbit polyclonal, 1:200; Torrey Pines; Houston, TX) overnight.
The sections were then incubated with 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF antibody (1:1600) overnight at 4°C and visualized as described previously [ 11 ] . Briefly, the sections were exposed to a goat anti-rabbit antiserum (Jackson; 1:50 in PBS) for 1 hour, followed by exposure to goat-PAP (Jackson; 1:100 in PBS).
Sections from the MPG, the IMG and the sympathetic chain were processed for double-immunofluorescence in order to visualize both MIF and tyrosine hydroxylase, as follows: Sections were exposed to 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF (1:200) and anti-tyrosine hydroxylase (sheep polyclonal antibody; 1:1000; Pel-Freez; Rogers, AR) overnight at 4°C.