Words similar to buffer

Example sentences for: buffer

How can you use “buffer” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.
To the pellet 1 ml of 2.5% glutraldehyde (made in 0.1 M sodium cacodylate buffer) was added and centrifuged at 1150 g for 10 min, supernatant was decanted.
The beads with the bound proteins were washed and then resuspended in 30 μl of kinase buffer [50 mM HEPES, pH 7.4, 2.5 mM EGTA, 10 mM β-glycerophosphate, 0.1 mM vanadate, 1 mM NaF, 1 mM DTT, 10 mM MgCl 2 , 20 μM ATP, 50 μCi γ- 32P-ATP (7000 Ci/mmol, ICN), and 1 μg histone H1].
Streptokinase activity was measured as described previously [ 46 ] . Briefly, aliquots of culture supernatants (100 μL) were mixed with either 1 μg of purified human plasminogen or buffer.
Cells were lysed in lysis buffer (20 mM HEPES, pH 7.4, containing 100 mM NaCl, 5 mM MgCl 2 , 5 mM EDTA, 0.5% Triton X-100 and 1 mM PMSF) with 1 × proteasome inhibitor cocktail (Boehringer Mannhein).