Words similar to buffer

Example sentences for: buffer

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The final result was approximately 50 μl of nucleic acid in elution buffer.
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.
Pellets were homogenized in 12 ml of ice-cold 50 mM Tris HCl buffer (pH 8.0) with a Tissumizer (Tekmar, Cincinnati, OH) for 20 sec at 75% of the maximum speed and the suspension was centrifuged at 47,000 × g for 10 min at 4°C.
Eluted fractions were evaluated by spectrophotometry and immunoblot analysis [ 42 ] . Fractions with the highest absorbance at 280 nm and the strongest reactivity by immunoblot were neutralized in 1 M Tris-HCl (pH 9.5) buffer and stored at 4°C.
For each set of heterodimers, 260 μg of p51 was mixed with 660 μg of p66 in the buffer containing 50 mM Tris HCl, pH 7.8, 60 mM KCl and 5 mM MgCl 2 . The rationale for using a 1:3 ratio of p51 to p66 was to saturate the His-tagged p51 with the non-tagged p66, ensuring heterodimer formation and eliminating excess p66 during IMAC purification.