Example sentences for: buffer

How can you use “buffer” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were washed with permeabilization buffer and then with staining buffer.

  • Total RNA was prepared from synovial tissue by an acid phenol method according to the procedure described by Chomczynski and Sacchi [ 31 ] . RNA (20 μg) was digested with five units of DNase in 1× reverse transcription buffer (Gibco/Life Technologies, Grand Island, NY, USA) containing eight units of RNase inhibitor (Gibco/Life Technologies, Grand Island, NY, USA) for 30 minutes at 37°C.

  • Immune complexes were washed five times with cold Triton X-100 lysis buffer.

  • Hybridization was carried out in the same buffer with a single stranded internally labeled 32P probe over night at 42°C.

  • Plates were placed on ice and incubation buffer aspirated.


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