Words similar to buffer

Example sentences for: buffer

How can you use “buffer” in a sentence? Here are some example sentences to help you improve your vocabulary:

Protein lysates from whole cells and tissues were prepared with a buffer, which contained 50 mM Tris, pH 7.5, 150 mM NaCl, 1% (v/v) Nonidet P40 (NP40), 2 mM 4-(2-aminoethyl)benzenesulphonyl fluoride, 1 μg/ml leupeptin, 2 μg/ml aprotinin and 2 μg/ml pepstatin.
Peptides and cells were prepared in Tris Buffered Saline Binding Buffer: 50 mM Tris, 120 mM NaCl, 0.2 mg/ml bacitracin, 20 μg/ml leupeptin, 20 μg/ml chymostatin, 0.1 % bovine serum albumin, pH 7.4.
They were then applied on a heparin-Sepharose column (bed volume 5 ml) that was equilibrated and run (flow rate 20 ml/h) with a buffer consisted of 50 mM sodium acetate, pH 5.6, containing 0.25 M NaCl and 0.01% Tween-20.
Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.
Plasmids were stored at -20 °C as a 1 μg μl -1stock solution in TE buffer (10 mM TRIS, 1 mM EDTA, pH 8).