Example sentences for: buffer

How can you use “buffer” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Equivalent amounts of protein were boiled in SDS sample buffer and separated by 10% SDS-PAGE and transferred onto nitrocellulose (Schleicher and Schuell).

  • Nuclear protein was then extracted from the purified nuclei with buffer A containing 1.0 M KCl.

  • Next, the cells were resuspended at 3 × 10 6cells/ml in ice cold sonication buffer (250 mM sucrose; 10 mM HEPES, pH 7.4; 1 mM EDTA; 5 mM MgCl 2 ; 1 mM DTT; 0.1 mM GTP; 1 μg/ml leupeptin, 1 μg/ml pepstatin A, and 1 μg/ml aprotinin; 1 mM PMSF) and sonicated for 10 seconds.

  • Steady-state kinetic studies were conducted with both mutants in buffer containing 100 μM ZnCl 2 to ensure that both Zn(II) binding sites were saturated in these studies.

  • After labeling, cells were washed and resuspended in RPMI 1640 supplemented with 1% fetal calf serum and 20 mM Hepes buffer.


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