Example sentences for: blocking

How can you use “blocking” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Despite the use of unlabeled polyA as a blocking agent, when microarray hybridization results obtained using probes derived from mRNA from two different sources are nearly the same, one cannot be certain that the similarity of the results is due to the similarity of the different mRNA source material, rather than to incomplete blockage of the polyA effect in both of the hybridizations.

  • The results obtained with ERK1/2 activation closely paralleled the results obtained with cell attachment to III1-C, except that the individual blocking agents were relatively more efficient at inhibiting ERK1/2 activation.

  • Total cellular RNA was extracted from CD14 + lung macrophages stimulated overnight with rIP-10 (500 ng/ml) in the presence or absence of blocking anti-CXCR3 antibodies (5 μg/ml, R&D Systems).

  • In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .

  • Additionally, the mutant confirms previous work that the two known LcrG functions (secretion blocking and LcrV interaction) are genetically separable [ 5 ] . Further experiments will more precisely delineate the region of LcrG required to block the type III secretion apparatus.


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