Example sentences for: blocking

How can you use “blocking” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, cells were first treated with a human TNFR1 blocking mAb, mAb225 (10 ug/ml), for at least 30 min at 37 C prior to the addition of agents.

  • In brief, after dewaxing, inactivating endogenous peroxidase activity and blocking cross-reactivity with normal serum (Vectastain Elite Kit; Vector, Burlingame, CA), the sections were incubated overnight at 4□ with a diluted solution of the primary antibodies (1:1000 for p53, 1:200 for iNOS and 1:100 for VEGF).

  • Immunoreactivity was visualized by indirect immunofluorescence as previously described [ 16 ] . The specificity of the immunoreaction was determined by preincubating the BMP-5 Ab with its specific blocking peptide (100 μg/ml) prior to reaction with the cultures.

  • Total MAPK levels were quantified using the ERK-1 antibody (C-16 epitope; 1:5,000 dilution in blocking solution), which was also obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

  • In blocking experiments, the supernatants were cultured with blocking mAbs specific for chemokines overnight at 4°C before assay.


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