Words similar to blocking
Example sentences for: blocking
How can you use “blocking” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, cells were first treated with a human TNFR1 blocking mAb, mAb225 (10 ug/ml), for at least 30 min at 37 C prior to the addition of agents.
In brief, after dewaxing, inactivating endogenous peroxidase activity and blocking cross-reactivity with normal serum (Vectastain Elite Kit; Vector, Burlingame, CA), the sections were incubated overnight at 4□ with a diluted solution of the primary antibodies (1:1000 for p53, 1:200 for iNOS and 1:100 for VEGF).
Immunoreactivity was visualized by indirect immunofluorescence as previously described [ 16 ] . The specificity of the immunoreaction was determined by preincubating the BMP-5 Ab with its specific blocking peptide (100 μg/ml) prior to reaction with the cultures.
Total MAPK levels were quantified using the ERK-1 antibody (C-16 epitope; 1:5,000 dilution in blocking solution), which was also obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
In blocking experiments, the supernatants were cultured with blocking mAbs specific for chemokines overnight at 4°C before assay.