Example sentences for: blocked

How can you use “blocked” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • When we incubated cells with cycloheximide, the accumulation of β-catenin by Wnt-3A was blocked; and Wnt targets, like MSX2, were not elevated (Figure 4).

  • Other states have blocked gambling--as Alabama did by defeating a lottery referendum this week--but this marks the first time in 50 years that a state has outlawed gambling and uprooted a living industry.

  • The filter was blocked with 3% BSA in TBS with 0.1% Tween 20 (T-TBS) for 30 min and incubated with the anti-phosphotyrosine antibodies at a dilution of 1:1000.

  • 5Bshows that LcrG expression blocked secretion in this strain in the presence of calcium and allowed secretion in its absence (Fig.

  • Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.


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