Words similar to blat

Example sentences for: blat

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We used regions of amino acid sequence conserved between the midasin genes in yeast and Schizosaccharomyces pombe to make a rough map of the gene onto the August 2001 freeze of the assembled human genome with the public domain Blat server at http://genome.ucsd.edu/index.html [ 54 55 ] . The resultant partial nucleotide sequence identified about four-fifths of the exons and was used to design the requisite PCR primers to verify all exon-intron boundaries in the gene by physical sequencing of appropriate PCR-amplified cDNA fragments.
Orthologous regions of the D. melanogaster genome corresponding to sequenced fosmids were identified using default parameters of BLAT [ 78] against a database made up of the Release 3 sequences [ 9].
These one-to-many associations caused by alternative splicing (and/or alternative poly(A) addition sites, see below) were confirmed by mapping the TCs or DTs to the mouse genome assembly using BLAT search at the University of California Santa Cruz (UCSC) genome browser [ 21].
We mapped the coding sequences to the chromosomal sequences by BLAT [ 14 ] . Then, for each gene, if the upstream intergenic region is greater than 3 kb, we retrieved the sequence upstream of ATG of length 3 kb.
This conclusion is supported by unique BLAT assignment of the probe sequence to the human genome [ 29 ] .