Example sentences for: bg-
How can you use “bg-” in a sentence? Here are some example sentences to help you improve your vocabulary:
BG-1 human ovarian adenocarcinoma cells were infected with a retroviral construct composed of an antisense 900 base-pair cDNA sequence of the amino-terminal region of BRCA1 . Three experiments (two of which were from independently made supernatants) showed that infection of pLXSN (vector alone) and BRCA1 antisense retroviral constructs into BG-1 cells yielded G418-resistant colonies at similar rates (titers ranged from 0.78 to 4.2 × 10 4colony-forming units/ml).
Decreased BRCA1 levels appeared to affect the ability of BG-1 cells to arrest growth or die in the absence of estrogenic growth-inducing conditions.
BRCA1 RNA and protein levels were successfully reduced in pooled and isolated subclones of antisense-infected populations of BG-1 cells.
BG-1 infected clones were tested for tumorigenicity by injection of cells (10 6cells in 0.1cm 250% matrigel; Collaborative Biomedical Products, Bedford, MA, USA) into subcutaneous sites in 6-week-old athymic Ncr-nude mice (NCI Animal Program, Bethesda, MD, USA) that were ovariectomized at approximately 4 weeks of age.
Therefore, we examined the effect of reduction of BRCA1 levels in BG-1 cells on the cellular response to hormone depletion as well as estrogen stimulation.