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Example sentences for: bgh

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This species is also not necessarily distinct from the 6,7+ETn transcript amplified by F1-BGH037, as both could be amplified from the same hypothetical RNA 6,7+60ETn,+37ETn,8.
RT-PCR analysis with this primer confirmed that while the F1-BGH037 transcript is only produced off the T Wis allele, the F3-R1 transcript is only produced off of the wild type T allele (Figure 2).
Given that the BGH037 primer is contained within the 60 nt of ETn read-through, this species is not necessarily distinct from the 6,7+ETn transcript amplified by F1-BGH037, as both could be amplified from the same hypothetical RNA 6,7+60ETn,8.
Primers used for RT-PCR and/or sequencing were: F1 (5'-GTCATCGCCCTACCCCAG-3'), R1 (5'-GTGTGCGTCAGTGGTGTGTAATG-3'), F2 (5'-CCCATTTGCTAAAGCCTTCCTTG-3'), R2 (5'-AGGCACTCCGAGGCTAGACCAG-3'), BGH037 (5'-ACGTTGCGAGCTGCTGCGGC-3'), and F3 (5'-CATAACGCCAGCCCACCTACTG-3').
The primers BGH052 and BGH037 amplify an ~180 base pair (bp) fragment from the T Wis insertion allele, while primers BGH052 and BGH039 amplify an ~220 bp fragment from the wild type allele.