Example sentences for: bgh
How can you use “bgh” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 embryos, PCR was performed on lysates of extraembryonic tissue as template, using primers BGH037 (5'-ACGTTGCGAGCTGCTGCGGC-3'), BGH039 (5'-ACCCATGTCAAACCCATCAG-3'), and BGH052 (5'-CCTATGCGGACAATTCATCTG-3'), in a similar reaction mix except for 1.0-1.
Given that the BGH037 primer is contained within the 60 nt of ETn read-through, this species is not necessarily distinct from the 6,7+ETn transcript amplified by F1-BGH037, as both could be amplified from the same hypothetical RNA 6,7+60ETn,8.
RT-PCR analysis with this primer confirmed that while the F1-BGH037 transcript is only produced off the T Wis allele, the F3-R1 transcript is only produced off of the wild type T allele (Figure 2).
This species is also not necessarily distinct from the 6,7+ETn transcript amplified by F1-BGH037, as both could be amplified from the same hypothetical RNA 6,7+60ETn,+37ETn,8.
The primers BGH052 and BGH037 amplify an ~180 base pair (bp) fragment from the T Wis insertion allele, while primers BGH052 and BGH039 amplify an ~220 bp fragment from the wild type allele.