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Example sentences for: bf-

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5.4D for type I [ 23 ] , SC-71 for type IIa [ 24 ] , and BF-F3 for type IIb [ 24 ] . The antibody useful for the identification of type IIx fibers, BF-35 [ 24 ] , stains all fibers except pure IIx fibers.
Subjectively, we saw no evidence of coexpression of fiber types I and IIa, but owing to the nature of antibody BF-35, which stains all except pure IIx fibers, we are unable to rule out the coexpression of IIx with any other isoform.
There were very rare fibers (less than 1%) that stained only lightly with BF-F3 and did not stain with BF-35.
For BF-35 we used a primary antibody dilution of 1:10, a 24 hour incubation, and a goat anti-mouse IgG rhodamine-conjugated secondary antibody.
Fibers were classified on the basis of the antibody that yielded the strongest fluorescent staining (or the absence of staining with BF-35 for pure IIx fibers).