Words similar to bfa

Example sentences for: bfa

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Cdc5 presumably impinges on the MEN by other means as well, because Cdc5 is required for maximal Dbf2 protein kinase activity even in the absence of Bfa1's obligate partner Bub2 [ 18 ] . Once activated, the MEN in turn sustains the transient Cdc14 release enabled by Cdc5 and other FEAR pathway components.
After 2 hours at 37°C, the reactions were stopped by heating to 80°C for 20 minutes and the reactions Bfa I, Dpn I and Rsa I as well as Dde I, Alu I and Hinf I were pooled, respectively.
5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
In a second step, the plasmids were digested with restriction enzymes recognizing 4 bp sequences (Alu I, Bfa I, Dde I Dpn I, Hinf I, or Rsa I) in six separate reactions (Fig.
Cdc5 also helps to activate the MEN by directly phosphorylating and disabling Bfa1 [ 29 ] , which is thought to inhibit Tem1 by serving as a GTPase-activating protein.