Words similar to bfa
Example sentences for: bfa
How can you use “bfa” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cdc5 also helps to activate the MEN by directly phosphorylating and disabling Bfa1 [ 29 ] , which is thought to inhibit Tem1 by serving as a GTPase-activating protein.
Because the putative Tem1 antagonists and mitotic checkpoint proteins Bub2 and Bfa1 negatively regulate exit from mitosis and cytokinesis in response to both DNA damage and spindle misorientation ( [ 32 ] ; reviewed in [ 33 ] ), we asked whether bypass of TEM1 -dependent regulation can cause chromosome instability.
fingerprint MCP-2, Bfa I, Hinf I, Rsa I, Dpn I, Dde I, Alu I) CTAGN 177-181 GANTCN 90-94 GTACN 3-7 GATCN 41-45 CTNAGN 123-127 AGCT were derived from the restriction fingerprints.
5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
Tem1 is thought to be kept inactive throughout early mitosis by the two-component GTPase-activating protein complex Bub2/Bfa1 which co-localizes with Tem1 at the spindle pole body [ 10 11 ] . When the mitotic spindle penetrates the mother-daughter neck, the Tem1-bound spindle pole body is translocated into the daughter cell where a cortical pool of Lte1 is thought to activate Tem1 [ 12 13 ] . In addition to this spatial regulation, the GTPase activity of Bfa1 is thought to be inactivated through its phosphorylation by Cdc5 [ 14 ] , but it remains unknown how this process is coordinated with the presumed juxtaposition of Lte1 and Tem1 in the daughter cell.
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