Words similar to bgl
Example sentences for: bgl
How can you use “bgl” in a sentence? Here are some example sentences to help you improve your vocabulary:
A full length, 1.2 kb IDO cDNA was amplified from IFN-γ stimulated RAW cells and cloned into pGEM T-Easy (Promega), using primers; 5' TAG CGG CCG CGT AGA CAG CAA TGG CAC TC 3' forward, 5' TAA GAT CTT ACA CTA AGG CCA ACT CAG 3' reverse, which contain Not I and Bgl II sites respectively.
pJC14 was a Hpa I- Bsp EI deletion (309 bp) of pJC10, and pJC15 was a Bgl II- Bsp EI deletion (150 bp) of pJC10.
Plasmid pBPhin2 was created by ligating the Bgl II-digested Rv2233-Rv2235 fragment into the Bam HI site of pBPhin.
In this PCR amplified product, there is no predicted Bgl1 restriction enzyme site in the corresponding ECaC1 sequence.
These primers yield a 259 bp PCR product with a specific restriction site that makes it possible to clearly distinguish between CaT1 and ECaC1 using the restriction enzymes Bgl1 (New England Biolabs).
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