Words similar to autogene

Example sentences for: autogene

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Purified, overlap PCR products and autogene vectors containing mutated promoters (e.g.
The T7 RNA polymerase gene was linked to a T7 RNA polymerase promoter, creating a so-called autogene [ 10 ] , whose activity can be initiated by the basal level expression of polymerase in the cell.
In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).
To address this problem, we have developed a combined in vitro / in vivo selection method based on T7 RNA polymerase autogene construct that allows large (10 3- 10 6) libraries of T7 RNA polymerase variants to be efficiently searched for specificity mutants.
2. Upon annealing, sticky ends were generated that were suitable for ligation into the pET/T7p/T7 autogene construct cleaved with BglII and XbaI.