Words similar to autogene
Example sentences for: autogene
How can you use “autogene” in a sentence? Here are some example sentences to help you improve your vocabulary:
In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).
To address this problem, we have developed a combined in vitro / in vivo selection method based on T7 RNA polymerase autogene construct that allows large (10 3- 10 6) libraries of T7 RNA polymerase variants to be efficiently searched for specificity mutants.
In constructing the autogene pool for the second selection, two stop codons were first introduced at amino acid positions 747 and 748 in the wild-type RNA polymerase gene to form pET/T7/T7stop.
Therefore, the fraction of colonies that were lost on replica plating to IPTG was assumed to be proportional to the accumulation of active autogene variants.
These initial studies showed that the wild-type autogene did not affect cellular viability for at least 2-3 hours after induction with IPTG.
Loading...