Example sentences for: autogene

How can you use “autogene” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The wild type autogene thus obtained (pET/T7p/T7) was transformed into strain HMS174 pLysS (Novagen, Madison, WI), the same cell line Studier et al.

  • The T7 RNA polymerase gene was linked to a T7 RNA polymerase promoter, creating a so-called autogene [ 10 ] , whose activity can be initiated by the basal level expression of polymerase in the cell.

  • In this study, we have developed a facile in vitro / in vivo selection methodology using a T7 RNA polymerase autogene to select polymerase variants with apparently altered promoter specificities.

  • In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).

  • Cells containing active autogene cease to grow when lifted to LB plates containing IPTG.


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