Example sentences for: autogene

How can you use “autogene” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).

  • To address this problem, we have developed a combined in vitro / in vivo selection method based on T7 RNA polymerase autogene construct that allows large (10 3- 10 6) libraries of T7 RNA polymerase variants to be efficiently searched for specificity mutants.

  • In constructing the autogene pool for the second selection, two stop codons were first introduced at amino acid positions 747 and 748 in the wild-type RNA polymerase gene to form pET/T7/T7stop.

  • Therefore, the fraction of colonies that were lost on replica plating to IPTG was assumed to be proportional to the accumulation of active autogene variants.

  • These initial studies showed that the wild-type autogene did not affect cellular viability for at least 2-3 hours after induction with IPTG.


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