Words similar to autogene

Example sentences for: autogene

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2. Upon annealing, sticky ends were generated that were suitable for ligation into the pET/T7p/T7 autogene construct cleaved with BglII and XbaI.
The wild type autogene thus obtained (pET/T7p/T7) was transformed into strain HMS174 pLysS (Novagen, Madison, WI), the same cell line Studier et al.
Purified, overlap PCR products and autogene vectors containing mutated promoters (e.g.
Oligos containing the promoter pool randomized between -8 and -11 positions were then cloned into pET/T7/T7stop to form the autogene construct with the promoter pool, pET/T7pp/T7stop.
In order to evaluate the effect of toxicity of autogene on the selection process, the effect of the wild-type autogene construct (pET/T7p/T7) on cell growth was studied before and after induction with IPTG (Figure 3).