Example sentences for: autofluorescence

How can you use “autofluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The images were taken in the red confocal channel to visualize the morphology of the tissues while eliminating the effects of autofluorescence.

  • However, one advantage of using rhod-2 is that its long excitation and emission wavelengths relative to other calcium sensitive dyes results in minimal filtering of excitation and emission light and autofluorescence [ 7 20 ] . At these visible range wavelengths light may penetrate between 0.3 and 0.4 mm into the myocardium [ 21 ] which may be significantly deeper than shorter wavelength dyes.

  • Autofluorescence and non-specific staining were blocked for 30 min in 0.1 M glycine in PBS, followed by 30 min in 1% Evans Blue in PBS to quench red blood cell autofluorescence.

  • In order to correct for non-specific increase in autofluorescence, "brightness" of cells treated with PMA alone (Table 1, column 1) was subtracted from "brightness" of cells treated with PMA+IFNg (Table 1, columns 2-4), e.g., group 05a value was subtracted from values of group 11a, 17a and 23a, etc.

  • Interestingly, this same pattern of autofluorescence was evident when the microglia were viewed with the TRITC filter configuration (not shown).


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