Words similar to assays

Example sentences for: assays

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Solution hybridization/RNase protection assays
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
In addition, BRCA1 can act as a transcriptional transactivator in yeast reporter assays [ 26, 27] and binds the RNA polymerase II holoenzyme, a component of the basal transcription machinery [ 25].
The Gal-β-box fusion failed to recruit the ERα, TRβ or RARβ LBDs in mammalian two-hybrid assays (Fig.
However, in another study, butorphanol was reported to be equipotent in its antinociceptive effect to that of morphine [ 32 ] . In our assays, we have observed that butorphanol and morphine are equally efficacious in inhibiting cAMP production, although butorphanol is more potent than morphine in this assay.