Words similar to assays
Example sentences for: assays
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The sensitivity of the gene expression system to varying doses of GS-E was determined by treating duplicate samples of clone MBT-2+RHeoSwitch-2+luc-5B3 with varying concentrations of GS-E for 24 or 48 hours and dual luciferase assays were performed on the cell lysates (Figure 3B).
Plasma and whole blood RNA (PAX tube isolators, Qiagen) samples were isolated from blood, and stored under appropriate conditions for subsequent assays.
Author 1 D. L. Sheridan carried out the design and construction of the transposons, conducted the biochemical assays for G-protein signaling, imaged the living cells, and drafted the manuscript.
A screen of a HeLa cell library yielded 56 colonies that tested positive in the two hybrid assays (growth on plates lacking histidine or uracil, lack of growth on plates containing 5-fluoroorotic acid; and induction of lacZ resulting in blue color when assayed with X-gal).
In two-hybrid assays, N-terminal deletions of ~36 % of BTBD1 (177 aa) or 43% BTBD2 (228 aa) did not eliminate binding to TOP1 (Fig.