Words similar to assaying
Example sentences for: assaying
How can you use “assaying” in a sentence? Here are some example sentences to help you improve your vocabulary:
PP5 activity was followed during the purification by assaying trypsin-stimulated pNPPase activity.
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
Testing specific genes by overexpression in transgenic flies is a candidate gene approach, meaning that the investigator must make an educated guess as to which genes are worth assaying by this relatively labor-intensive method.
PP5 activity was followed during the purification by assaying for trypsin-stimulated phosphohydrolase activity at 37°C vs. p-nitrophenyl phosphate in 200 μl reactions containing: 25 mM tris pH 8.3, 25 mM MgCl 2 , 1 mM DTT and 10 mM p-nitrophenyl phosphate.
Progress has been made recently in assaying RNAi effects in both plant [ 66 ] and animal [ 67 ] parasitic nematodes.
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