Words similar to assaying
Example sentences for: assaying
How can you use “assaying” in a sentence? Here are some example sentences to help you improve your vocabulary:
Progress has been made recently in assaying RNAi effects in both plant [ 66 ] and animal [ 67 ] parasitic nematodes.
While the anti-tumor effect of the combinatorial treatment does not completely resolve tumor burden and the mice eventually succumb, it is important to recognize that the Rip1-Tag2 mice are a very stringent system for assaying immunotherapies because of the multifocal nature of tumor progression in these mice.
A reporter construct containing the basal promoter of the rat RIIβ 5'-flanking region (-394 to -123) upstream of a CAT-reporter gene (pCATbasic; Promega, Madison, WI) was used for assaying RIIβ promoter activity [ 18 ] . Expression vectors for USF1, USF2a and USF2b inserted 3' of the CMV promoter in pCR™3 (Invitrogen, Groningen, The Netherlands) were used to overexpress USF isoforms [ 12 ] . C/EBPβ LAP (full-length) and LIP (DNA-binding domain) expression vectors created in pCMV™3 (Stratagene, La Jolla, CA) were appreciated gifts from Dr. Shizua Akira (Hyogo College of Medicine, Hyogo, Japan).
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
Testing specific genes by overexpression in transgenic flies is a candidate gene approach, meaning that the investigator must make an educated guess as to which genes are worth assaying by this relatively labor-intensive method.
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