Words similar to assay
Example sentences for: assay
How can you use “assay” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were plated in 96-well plates (human primary RPE: 10,000 cells/well, ARPE-19: 15,000-20,000 cells/well, unless otherwise stated) overnight, fed with serum-free medium or treated with testing agents (prepared in serum-free medium) the next day followed by H 2 O 2 treatment for a day, then the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [ 27 ] . Culture medium was removed after treatment and then 100 μl MTT solution (100 μg/ml prepared in DMEM) was added to each well.
Beta-glucuronidase binding assay
For saturation experiments, the resulting pellet (a crude particulate membrane fraction) was resuspended in 200 volumes of 25 mM NaPO 4 buffer (pH 7.4) and re-homogenized for use in the radioligand binding assay.
Using this assay, we determined a p27 half-life of 4 h in control cells, which was increased to more than 6 h in MIB treated cells (Fig.
Similarly, only 2 apoptotic cells were detected in the developing SC, TM, iris and ciliary body of B6 eyes using a cell death assay on sections collected throughout entire eyes.