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Example sentences for: assayed
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Reduction of virus yield was observed with all compounds assayed (Table 4), with most compounds resulting in 95 - 99% reduction at 50 μg/mL.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
Tag immortalized human tracheal epithelial (HTE) cells grown at different densities and assayed for cytokeratins 6 and 18 showed a density dependent decrease in cytokeratin immunoreactivity and a concomitant increase in %G 1 both in normal growth medium and in differentiation medium (Frisa & Jacobberger, unpublished).
When assayed side-by-side, the basal specific activities for recombinant Ppt1p and PP5 were similar toward the artificial substrates 32P-MBP and 32P-casein, respectively (Fig.
All procedures were done at 4°C and the protein concentrations of samples were assayed using the Coomassie Blue method.
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