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Example sentences for: assayed

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Assays of invertase activity were performed with 25 μl of each sample according to [ 70 71 ] . The activity of each mutant was normalized to the activity of the parental strain assayed at the same time.
Deletion of the cryptochromes disinhibits the transcriptional activation of CLOCK:BMAL1 and NPAS2:BMAL1 target genes, resulting in increased levels of their transcripts in the SCN, liver, and retina [ 20 23 25 ] . We assayed the expression in the brain of three genes that are known targets of cryptochrome mediated transcriptional inhibition: albumin D-binding protein ( dbp [ 26 ] ), period (per)1 , and per2 [ 20 23 27 ] , in the middle of the daily light period.
The use of lymphoblastoid cell lines, while not all clonally derived, expanded the scope of genes to be assayed beyond those expressed in skin fibroblasts.
24 cells as described previously [ 44 ] . To prepare lipid suspension, phosphatidylserine and phosphatidylinositol (1:1) were sonicated in 25 mM 3-(N-morpholino)propanesulfonic acid (MOPS; pH 7.0), 1 mM EGTA, 1 mM Na 3 VO 4 . Cell extracts were assayed in 25 mM MOPS (pH 7.5), 5 mM MgCl 2 , 1 mM EGTA, 1 mM Na 3 VO 4 , 130 μM ATP, 20 μCi [ 32P]-ATP (3000 Ci/mmol) and 0.2 mg/ml sonicated lipids.
It was acknowledged that MJ0107 (a member of COG0294) could be identified as a possible homolog of DHPS by sequence-based methods, and this protein was assayed for dihydropteroate synthase activity, but none was detected [ 25].