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Example sentences for: appppn

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Larger increases in AppppN concentrations have been seen with disruption of Apa1 and Apa2, the diadenosine tetraphosphate phosphorylases in S. cerevisiae [ 34 35 ] , indicating that they have a more significant role in controlling AppppN levels than does Hnt2.
As shown in Figure 3, ApppN and AppppN levels are higher in hnt2 strains than in cells with a functional HNT2 gene, and were not significantly elevated by 2 mM CdCl 2 . However, when cells were heat shocked, as shown in Figure 4, ApppN and AppppN levels increased substantially in cultures with every HNT2 genotype (absence, presence or active site mutation).
Hypotonic, hypertonic and caffeine treated media produced no increase in AppppN (not shown).
At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.
Haploid segregants from three complete tetrads were cultured for 24, 48 and 72 hours in SDC medium, lysed, and levels of ApppN and AppppN were determined.