Words similar to appppa
Example sentences for: appppa
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However, deletion of lysU had no effect on heat-shock inducible AppppA accumulation [ 37 ] . The KRS1 gene [ 38 ] encoding cytosolic lysyl tRNA synthetase was cloned into multicopy plasmid pRS423 [ 39 ] to generate plasmid pM1.
On the basis of observations [ 12 13 ] discussed above, one would expect AppppA with two phosphorothioadenosyl substituents to be a slow substrate with a relatively low K m . However, carbon in place of the β phosphorus (for example in 6b ) would make the leaving group the alkoxide - OCH 2 -CH(OH)-CH 2 O-phosphorothioadenosyl rather than α-thio ATP.
On the basis that Fhit binds diadenosine polyphosphates with one AMP group buried and the other adenosine solvent-exposed in a specific conformation [ 12 ] , effective tri and tetrapodal inhibitors such as 12b, 13b and HC(ADP) 3 [ 15 ] may exist in solution predominantly in a conformation that resembles Fhit-bound AppppA.
Earlier, Blackburn and co-workers made "supercharged" methane-trisphosphonic acid AppppA analogs containing a central carbon from which three adenylate or phosphate moieties are bonded [ 15 ] . In work presented here, tripodal inhibitors 15a and 17a showed, respectively, micromolar and submicromolar efficacies while the phosphorothioate counterparts 15b and 17b showed 100 nM efficacy.
Phosphorothioate analogs 6b and 12b that preserve the α-β bridging oxygen but substitute carbon for the β phosphorus, achieving 65 to 75-fold binding advantages over AppppA, are expected to be critical for cell biological characterization.
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