Words similar to apppbodipy
Example sentences for: apppbodipy
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Inhibitors, at approximately 4,2, 1, 0.5 and 0.25 times K i value, were added to assays of Fhit with ApppBODIPY [ 10 ] . K i values were obtained by calculating the inhibitor concentration-dependence in reduction of k cat / K m (apparent) as earlier described [ 10 ] .
Sodium salts of each compound were titrated into assays of purified Fhit [ 16 ] with 1.8 μM fluorescent substrate, Appp-S-(4-4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl)methylaminoacetyl (ApppBODIPY) [ 10 ] , and competitive K i values were obtained by calculating the inhibitor concentration-dependence in reduction of k cat / K m (apparent) for substrate hydrolysis [ 10 ] . As shown in Table 1, inhibitors 2b and 3b with two-carbon diol linkers were either only as inhibitory as the natural substrate 1 or substantially less so.