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Example sentences for: apppn

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In the case of disruption of the Fhit and Hnt2-homologous aph1 gene in S. pombe , which encodes an enzyme relatively specific for a AppppA [ 20 ] , a 290-fold increase in AppppA concentration was observed [ 22 ] . Our data indicate that Hnt2 hydrolyzes ApppN and AppppN in vivo in budding yeast and that an intact adenine biosynthetic pathway is required for high-level synthesis and accumulation of adenylylated dinucleoside polyphosphates.
Similarly, among the HNT2 samples, the heat shocked samples showed increased ApppN levels compared with control-treated cells while CdCl 2 and other treated samples showed no significant changes.
Thus, deletion of hnt2 afforded a 48-fold increase in ApppN in ade2 mutants, consistent with an earlier report of a 31-fold effect [ 13 ] , but a 211-fold increase in ADE2 strains.
Among the hnt2 samples, only heat shocked samples showed evidence of ApppN levels higher than the levels in nonstressed hnt2 cells.
An earlier report demonstrated that disruption of HNT2 was tolerated by haploid yeast strains without an effect on growth and that ApppN and AppppN accumulate 30 and 3-fold, respectively on account of the hnt2 deletion [ 13 ] . Because those data were obtained by random spore analysis, we considered it important to test whether elevated dinucleoside polyphosphate levels co-segregate with hnt2 disruption in all tetrads examined and whether any other commonly used genetic markers affect dinucleoside polyphosphate levels.