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Example sentences for: apppn

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At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.
As with other experiments, AppppN levels were lower than ApppN levels in all cases.
In ADE2 strains examined herein, deletion of HNT2 increased levels of ApppN and AppppN by factors of approximately 200 and 4, respectively.
Strains disrupted for hnt2 had ApppN levels of approximately 6 to 43 μM after one day of culture, rising to approximately 30 to 300 μM and 50 to 350 μM after two and three days of culture, respectively.
Determination of ApppN and AppppN concentrations revealed that ApppN levels are substantially higher in hnt2 mutants than in isogenic wild-types at all time points and that plasmids conferring multiple copies of KRS1 did not increase ApppN or AppppN levels at any culture time point (Table 5).