Words similar to anti-phospho-p
Example sentences for: anti-phospho-p
How can you use “anti-phospho-p” in a sentence? Here are some example sentences to help you improve your vocabulary:
Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.
The primary antibodies used in this set of experiments were from New England Biological (anti-phospho-c-jun at serine 73, used at 1:1000) or Santa Cruz (anti-c-jun, used at 1:3000; anti-phospho-c-jun at serine 63, used at 1:1000, anti-JNK, used at 1:3000; anti-ERK 1/2, used at 1:3000; anti-phospho-ERK, used at 1:2000; anti-p38, used at 1:3000; anti-phospho-p38, used at 1:1000).
Cells were then plated onto precoated dishes and processed for immunoblotting with anti-phospho-p44/42 MAPK antibody as described above.
Alternatively, after heparitinase digestion cells were lysed directly in SDS sample buffer and analyzed by immunoblotting with the 3G10 mAb (essentially as described above for anti-phospho-p44/42 MAPK immunoblotting).
To further investigate whether the MEK1/ERK pathway played a role in regulating AA release independent of Ser 505phosphorylation, we treated cells with anisomycin, which activates the MAPK homolog p38, but not the MEK1/ERK pathway [ 32 ] . Activation of p38 in response to anisomycin treatment was analyzed by immunoblotting using an anti-phospho-p38 antibody (Fig.