Example sentences for: anti-phospho-p

How can you use “anti-phospho-p” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.

  • To further investigate whether the MEK1/ERK pathway played a role in regulating AA release independent of Ser 505phosphorylation, we treated cells with anisomycin, which activates the MAPK homolog p38, but not the MEK1/ERK pathway [ 32 ] . Activation of p38 in response to anisomycin treatment was analyzed by immunoblotting using an anti-phospho-p38 antibody (Fig.

  • The primary antibodies used in this set of experiments were from New England Biological (anti-phospho-c-jun at serine 73, used at 1:1000) or Santa Cruz (anti-c-jun, used at 1:3000; anti-phospho-c-jun at serine 63, used at 1:1000, anti-JNK, used at 1:3000; anti-ERK 1/2, used at 1:3000; anti-phospho-ERK, used at 1:2000; anti-p38, used at 1:3000; anti-phospho-p38, used at 1:1000).

  • Alternatively, after heparitinase digestion cells were lysed directly in SDS sample buffer and analyzed by immunoblotting with the 3G10 mAb (essentially as described above for anti-phospho-p44/42 MAPK immunoblotting).

  • Cells were then plated onto precoated dishes and processed for immunoblotting with anti-phospho-p44/42 MAPK antibody as described above.


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