Words similar to anti-phospho-

Example sentences for: anti-phospho-

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Work in our laboratory has shown that recognition of ERK by anti-phospho-ERK antibodies correlates with an increase in ERK activity [ 21 30 31 ] . Interestingly, the anti-phospho-ERK immunoblots revealed that ERKs were constitutively activated in untreated, quiesced MDCK cells and activation was not enhanced further by ATP or IONO (Fig.
After washing three times (including a 30 min time in cold PBS for the first wash), the cells were incubated with rabbit anti-phospho-STAT3 (Biosource), 1 μg Ab/10 6cells in 100 μl buffer.
The primary antibodies used in this set of experiments were from Santa Cruz (anti-ERK 1/2, used at 1:5000; anti-phospho-ERK, used at 1:1000; anti-PPARγ, used at 1:2000) or from Calbiochem (anti-4-HNE, used at 1:2,000).
Phospho-ERK was detected by mouse anti-phospho-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, used at 1:1500) and the Vectastain ABC kit (Vector Lab.
Protein (250 μg) from the control-infected or HIV-1-infected podocytes on day 14 of the differentiation protocol was also analyzed by western blot for total pRb (ECL detection of a 1:1000 dilution of rabbit anti-Rb, Santa Cruz Biotechnology) and phospho-Rb (Ser780) (ECL detection of a 1:500 dilution of rabbit anti-phospho-Rb (Ser780) antibody binding, Cell Signaling).