Words similar to anti-phosphorylated
Example sentences for: anti-phosphorylated
How can you use “anti-phosphorylated” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine which key pathways were activated, Western blot analyses of phosphorylated or cleaved proteins in E15+2 CONT and SN50-treated explants were conducted as previously described [ 5 ] . For this set of experiments, we first determined the specificity for each of the following antibodies purchased from Cell Signaling Technology (Beverly, MA) using E15 and E17 SMG homogenates: anti-phosphorylated Erk1/2 [phospho-p44/42 MAP kinase (Thr202/Tyr204)] antibody, anti-phosphorylated c-Raf(Ser259) antibody, anti-cleaved Caspase 3 (D 175) antibody, and anti-cleaved PARP (D214) antibody.
Cells were fixed with 4% paraformaldehyde, blocked with BSA, immunostained using anti-phosphorylated ERK1/2, and visualized using HRP-conjugated secondary antibody and bisbenzamidine substrate.
To quantitate differences in activated (phosphorylated) p53 protein, 3 SN50 and control E15 + 2 explants were sectioned, preincubated with unlabeled goat-anti mouse IgG as previously described [ 9 ] and sequentially incubated with a monoclonal anti-phosphorylated p53 (Ser15) antibody (Cell Signaling Technology, Beverly, MA), biotin-labeled goat anti-mouse IgG, and HRP-labeled SA (Zymed Laboratories, South San Francisco, CA), and counterstained with hematoxylin.
Anti-phosphorylated IκB-α polyclonal antibody, IκB monoclonal antibody and chemiluminescent detection reagents were from Cell Signaling Technology (Beverly, MA, USA).
Anti-ERK1/2 (p44/p42) polyclonal antibody, anti-phosphorylated ERK1/2 polyclonal antibody, and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG were purchased from New England Biolabs (Beverly, MA, USA).
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