Example sentences for: anti-phosphotyrosine

How can you use “anti-phosphotyrosine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Btk:ER itself was inducibly phosphorylated upon 4-HT treatment, as measured by anti-phosphotyrosine blotting and by a phosphospecific antiserum that recognises the Y223 site of Btk autophosphorylation.

  • The membranes were incubated with the appropriate primary antibody at 1/2000 (anti-Rlk and 4G10, anti-phosphotyrosine) or 1:1000 (anti-Fyn) dilution overnight at 4°C or 1-2 hours at room temperature.

  • It was possible that more Syk in the immunoprecipitate was required for detection by the anti-phosphotyrosine antibody.

  • The anti-phosphotyrosine immunoblot used is a relatively crude measure of Syk kinase activity in vivo . The possibility remained that any specific effects of constitutive Syk on its downstream targets might be masked by the combined effects of the other tyrosine kinases activated in response to BCR signaling.

  • An anti-phosphotyrosine antibody conjugated to horseradish peroxidase (SC7020, Santa Cruz Biotechnology) was used for the detection of phosphotyrosine-containing proteins by immunoblotting.


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