Example sentences for: anti-phosphotyrosine

How can you use “anti-phosphotyrosine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We also assessed the global pattern and levels of induced tyrosine phosphorylation of cell proteins by using a generalized anti-phosphotyrosine reagent (RC-20).

  • Transient expression of the Src*/Cas(SD) in Cos-7 cells followed by analysis of total cell lysates using anti-phosphotyrosine antibodies demonstrated that while the attenuated Src kinase domain alone did not increase total cellular tyrosine phosphorylated proteins as expected, expression of the Src*/Cas(SD) fusion resulted in tyrosine phosphorylation of a protein of the expected size of the chimera, as well as other proteins (Fig.

  • It was possible that more Syk in the immunoprecipitate was required for detection by the anti-phosphotyrosine antibody.

  • The membranes were incubated with the appropriate primary antibody at 1/2000 (anti-Rlk and 4G10, anti-phosphotyrosine) or 1:1000 (anti-Fyn) dilution overnight at 4°C or 1-2 hours at room temperature.

  • The anti-phosphotyrosine immunoblot used is a relatively crude measure of Syk kinase activity in vivo . The possibility remained that any specific effects of constitutive Syk on its downstream targets might be masked by the combined effects of the other tyrosine kinases activated in response to BCR signaling.


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