Words similar to anti-phosphotyrosine
Example sentences for: anti-phosphotyrosine
How can you use “anti-phosphotyrosine” in a sentence? Here are some example sentences to help you improve your vocabulary:
An anti-phosphotyrosine antibody conjugated to horseradish peroxidase (SC7020, Santa Cruz Biotechnology) was used for the detection of phosphotyrosine-containing proteins by immunoblotting.
Transient expression of the Src*/Cas(SD) in Cos-7 cells followed by analysis of total cell lysates using anti-phosphotyrosine antibodies demonstrated that while the attenuated Src kinase domain alone did not increase total cellular tyrosine phosphorylated proteins as expected, expression of the Src*/Cas(SD) fusion resulted in tyrosine phosphorylation of a protein of the expected size of the chimera, as well as other proteins (Fig.
It was possible that more Syk in the immunoprecipitate was required for detection by the anti-phosphotyrosine antibody.
Cell lysis, Immunoprecipitation, SDS-PAGE, and Western blotting were performed exactly as described [ 48 ] . Tyrosine phosphorylation of chimeras and other substrates were determined following immunoprecipitation using the indicated antibodies and probing with RC20-HRP anti-phosphotyrosine antibodies.
The membranes were incubated with the appropriate primary antibody at 1/2000 (anti-Rlk and 4G10, anti-phosphotyrosine) or 1:1000 (anti-Fyn) dilution overnight at 4°C or 1-2 hours at room temperature.