Words similar to anti-phosphotyrosine

Example sentences for: anti-phosphotyrosine

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An anti-phosphotyrosine antibody conjugated to horseradish peroxidase (SC7020, Santa Cruz Biotechnology) was used for the detection of phosphotyrosine-containing proteins by immunoblotting.
The anti-phosphotyrosine immunoblot used is a relatively crude measure of Syk kinase activity in vivo . The possibility remained that any specific effects of constitutive Syk on its downstream targets might be masked by the combined effects of the other tyrosine kinases activated in response to BCR signaling.
Furthermore, immunoprecipitation of the δ-OR, followed by immunoblotting using anti-phosphotyrosine antibodies, revealed that these inhibitors did not attenuate tyrosine phosphorylation of δ-ORs after DSLET treatment (Figure 9).
It was possible that more Syk in the immunoprecipitate was required for detection by the anti-phosphotyrosine antibody.
Cell lysis, Immunoprecipitation, SDS-PAGE, and Western blotting were performed exactly as described [ 48 ] . Tyrosine phosphorylation of chimeras and other substrates were determined following immunoprecipitation using the indicated antibodies and probing with RC20-HRP anti-phosphotyrosine antibodies.