Example sentences for: anti-phosphotyrosine

How can you use “anti-phosphotyrosine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Transient expression of the Src*/Cas(SD) in Cos-7 cells followed by analysis of total cell lysates using anti-phosphotyrosine antibodies demonstrated that while the attenuated Src kinase domain alone did not increase total cellular tyrosine phosphorylated proteins as expected, expression of the Src*/Cas(SD) fusion resulted in tyrosine phosphorylation of a protein of the expected size of the chimera, as well as other proteins (Fig.

  • Anti-Fyn (FYN3) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phosphotyrosine (4G10) from Upstate Biotechnologies (Lake Placid, New York).

  • We also assessed the global pattern and levels of induced tyrosine phosphorylation of cell proteins by using a generalized anti-phosphotyrosine reagent (RC-20).

  • The membranes were incubated with the appropriate primary antibody at 1/2000 (anti-Rlk and 4G10, anti-phosphotyrosine) or 1:1000 (anti-Fyn) dilution overnight at 4°C or 1-2 hours at room temperature.

  • Cell lysis, Immunoprecipitation, SDS-PAGE, and Western blotting were performed exactly as described [ 48 ] . Tyrosine phosphorylation of chimeras and other substrates were determined following immunoprecipitation using the indicated antibodies and probing with RC20-HRP anti-phosphotyrosine antibodies.


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