Words similar to anaphase
Example sentences for: anaphase
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To evaluate whether the net1(7m) and net1(19m') phosphosite mutants have an impact on FEAR, we analyzed Cdc14 localization in synchronous populations of dbf2-1 , net1(7m) dbf2-1, and net1(19m') dbf2-1 cells following release from an α-factor block in G1 phase at 25°C to α-factor-free medium prewarmed to 37°C (nonpermissive temperature for dbf2-1 ). As shown in Figure 6D, the percentage of cells that completely released Cdc14 during early anaphase (spindle length 0-8 μm) was reduced by ~50% in net1(7m) and ~25% in net1(19m') cells compared with wild-type cells.
Oocytes with premature anaphase, polyploidy, or structural chromosome aberrations were not found.
Wild type cells with short spindles were in pre-anaphase and showed focal nucleolar Cdc14 staining, and those with elongated mitotic spindles were in late anaphase and showed diffused Cdc14 staining (Figure 1, Rows 1 and 4; [ 8 9 ] ). In contrast, cdc5-1 cells uniformly exhibited focal Cdc14 staining whether they were grown exponentially at permissive temperature (25°C) or arrested in late mitosis at nonpermissive temperature (33°C) (Figure 1, Rows 2 and 5).
These include complexes involved in anaphase promotion, transcription repression, protein import, and molecular chaperoning [ 9 10 ] . Yeast two-hybrid analysis and in vitro interaction studies have shown that the TPR domain of PP5 interacts with the TPR domains of CDC16 and CDC27, which may be important in recruiting PP5 to the anaphase promoting complex (APC) and to the mitotic spindles during cell division (38).
Our analysis of Cdc5's role in release of Cdc14 from the RENT complex revealed that Cdc5 is required for disassembly of RENT in late anaphase, that Cdc5 can provoke premature disassembly of RENT in pre-anaphase cells, and that Cdc5 controls (at least in part) the phosphorylation state of Net1 during anaphase.
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