Example sentences for: anaphase

How can you use “anaphase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.

  • If Cdc5 directly promotes disassembly of RENT in vivo by a mechanism similar to that observed in vitro , then a Net1 mutant that cannot be phosphorylated by Cdc5 should not allow release of Cdc14 from the nucleolus in anaphase cells.

  • Both transcriptional and post-translational controls have been implicated in the operation of the Mitotic Exit Network and the regulation of the anaphase/telophase -> G1 transition [ 40 7 19 41 ] . To address whether the portion of the MEN that acts downstream of CDC15 can trigger a key aspect of the anaphase/telophase -> G1 transition (i.e.

  • The net1(19m') allele also showed a synthetic growth defect when combined with dbf2-1 . These observations imply that some of the residues mutated in net1(19m') represent in vivo phosphorylation sites that influence the MEN-dependent release of Cdc14 during anaphase.

  • To do this, we modeled the cell in three shapes: a sphere at early anaphase, a cylinder with two spherical caps at late anaphase, and a dumbbell with spherical cap domains connected by a cylindrical intercellular bridge.

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