Words similar to anaphase
Example sentences for: anaphase
How can you use “anaphase” in a sentence? Here are some example sentences to help you improve your vocabulary:
By contrast, Cdc14 was released from the nucleolus in the great majority of wild type cells by late anaphase.
Following anaphase I, a probability exists that both homologues and sister chromatids could randomly assort to the same pole and result in SC and aneuploid MII oocytes.
In support of this possibility, Spo11 of mice colocalizes extensively along homologously synapsed chromosomes during pachytene and dissociates when the homologs segregate from one-another during anaphase of MI [ 25 ] .
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
Although Cdc5 activity (1) is necessary to free nucleolar Cdc14 in late mitosis, (2) can elicit ectopic release of Cdc14 in pre-anaphase cells when overproduced, (3) regulates the phosphorylation state of Net1 during anaphase, and (4) can disassemble native and recombinant RENT complexes in vitro by phosphorylating Net1, a combination of in vivo and in vitro phosphosite mapping and mutagenesis experiments suggest that Cdc5 mediates exit from mitosis primarily by phosphorylating targets other than Net1.
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