Words similar to anaphase
Example sentences for: anaphase
How can you use “anaphase” in a sentence? Here are some example sentences to help you improve your vocabulary:
, not all Cdc5 phosphorylation sites on Net1 were mapped and mutated), we evaluated whether the net1 phosphosite-mutants exhibited a synthetic growth phenotype when combined with msd2-1 (a mutant allele of CDC5 ). This experiment was based on the idea that a reduction in phosphorylation sites on Net1 coupled with a reduction in Cdc5 activity might synergize to prevent efficient release of Cdc14 during anaphase, resulting in failure to exit mitosis.
Release of Cdc14 from the nucleolus in late anaphase requires Cdc5
The origin of single, unpaired chromatids in MII oocytes has been proposed to result from premature separation of sister chromatids prior to anaphase I [ 56 57 ] . The possibility exists that both SC and aneuploidy may have originated from PCS of homologues and sister chromatids during the MAT of meiosis I. Upon removal of MG-132 from the culture media during meiosis I and the subsequent resumption of OM and proteasomal activity, faulty kinetochore-microtubule attachment accompanied by degradation of cohesion proteins could conceivably lead to PCS of homologues and sister chromatids.
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
Mutant cdc15-2 cells were uniformly arrested in late anaphase/telophase by incubation at the restrictive temperature 37°C.
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