Example sentences for: aminoallyl-d

How can you use “aminoallyl-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The aminoallyl-dUTP labeled DNA templates are in turn coupled with Cy dyes.

  • The use of aminoallyl-dUTP in PCR amplification of the hybridized DNA template is not recommended.

  • With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.

  • RNA was reverse transcribed into cDNA using random hexamer primers in the presence of 5-aminoallyl-dUTP; Cy3 and Cy5 dyes were covalently coupled to the incorporated aminoallyl linkers in a subsequent labeling reaction.

  • Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.

How many words do you know? Try our free vocabulary size test!


Search for example sentences

Loading Loading...