Example sentences for: aminoallyl

How can you use “aminoallyl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA).

  • One approach to low specimen RNA input has been to use indirect labelling techniques to increase fluorescence signal intensity, such as with aminoallyl nucleotides.

  • RNA was reverse transcribed into cDNA using random hexamer primers in the presence of 5-aminoallyl-dUTP; Cy3 and Cy5 dyes were covalently coupled to the incorporated aminoallyl linkers in a subsequent labeling reaction.

  • As first-strand cDNA synthesis is identical for both samples, incorporation of nucleotides with an aminoallyl linker followed by the covalent coupling reaction to Cy dyes reduces bias for incorporation of one dye over the other by the reverse transcriptase.

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