Words similar to affinity-purified

Example sentences for: affinity-purified

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The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).
A band for RIIβ was detected in contrast to a report of no RIIβ detection in soleus or extensor digitorum longus muscles [ 18 ] , which might be explained by the presence of nerve fibers or adipocytes wherein RIIβ is prevalent [ 27 ] . The blot for the affinity-purified D-AKAP1 antibody is shown because the protein-A purified antibody displayed additional, unexplained bands; however, no difference in fluorescence labeling was observed when using antibodies purified either way.
Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.
Mouse monoclonal Ab 5B88 to β-galactosidase (GIBCO) was used at a concentration of 2.5 g/ml, followed by rhodamine-conjugated, affinity-purified goat anti-mouse IgG antibody (Boehringer Mannheim, Indianapolis, IN).
Pip1p and Pcu1p were detected with affinity-purified rabbit antisera.