Example sentences for: affinity-purified

How can you use “affinity-purified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Pip1p and Pcu1p were detected with affinity-purified rabbit antisera.

  • The rabbit antiserum obtained was affinity-purified by passage over a Sulfolink column (Pierce, Rockford, IL, USA) to which disulfide linkage of the peptide had been used to immobilize the peptide.

  • Finally, the data presented on MCM2 expression by IHC were obtained with affinity-purified polyclonal rabbit antibodies.

  • Mouse monoclonal Ab 5B88 to β-galactosidase (GIBCO) was used at a concentration of 2.5 g/ml, followed by rhodamine-conjugated, affinity-purified goat anti-mouse IgG antibody (Boehringer Mannheim, Indianapolis, IN).

  • The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).

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