Words similar to affinity-purified

Example sentences for: affinity-purified

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The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).
The affinity-purified polyclonal antibodies against threonine558-phospho-moesin were prepared as described previously [ 43].
The rabbit antiserum obtained was affinity-purified by passage over a Sulfolink column (Pierce, Rockford, IL, USA) to which disulfide linkage of the peptide had been used to immobilize the peptide.
Following incubation in blocking buffer (10 mM Tris-HCl, pH 7.5, containing 10% non-fat dry milk, 100 mM NaCl, and 0.1% Tween 20), the membranes were probed with an affinity-purified rabbit polyclonal IgG directed against caspase-8 (Caspase-8 p20, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1:200 dilution in blocking buffer, overnight at 4°C.
2 -δ protein G-purified guinea pig antibody #1 [ 13], or anti-β 1a affinity-purified sheep #6 antibody [ 44], or with anti-γ 1 affinity-purified guinea pig #16 antibody and the appropriate secondary antibodies [ 11].