Words similar to affinity-purified

Example sentences for: affinity-purified

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Affinity-purified rabbit antiserum raised against a recombinant chicken α-actinin fragment detected picogram amounts of the immunizing amino-terminal protein fragment (Fig.
A band for RIIβ was detected in contrast to a report of no RIIβ detection in soleus or extensor digitorum longus muscles [ 18 ] , which might be explained by the presence of nerve fibers or adipocytes wherein RIIβ is prevalent [ 27 ] . The blot for the affinity-purified D-AKAP1 antibody is shown because the protein-A purified antibody displayed additional, unexplained bands; however, no difference in fluorescence labeling was observed when using antibodies purified either way.
Affinity-purified polyclonal antibody (Ab) specific for BMP-5, the blocking peptide for the BMP-5 Ab, and the recombinant human BMP-RIA-Fc chimera were purchased from Research Diagnostics (Flanders, NJ).
As shown in Figure 4, affinity-purified mactinin antisera reacted with several 30-40 kDa proteins, including 31 kDa mactinin in each of the samples tested.
Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.