Words similar to affinity-purified
Example sentences for: affinity-purified
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Mouse monoclonal Ab 5B88 to β-galactosidase (GIBCO) was used at a concentration of 2.5 g/ml, followed by rhodamine-conjugated, affinity-purified goat anti-mouse IgG antibody (Boehringer Mannheim, Indianapolis, IN).
Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.
2 -δ protein G-purified guinea pig antibody #1 [ 13], or anti-β 1a affinity-purified sheep #6 antibody [ 44], or with anti-γ 1 affinity-purified guinea pig #16 antibody and the appropriate secondary antibodies [ 11].
Affinity-purified anti-α 2 β 1 integrin (MAB1988), anti-α v β 3 integrin (LM609), anti-MMP2 (MAB13405), and anti-VE-cadherin (MAB1989) monoclonal antibodies (mAbs) were purchased from Chemicon Intl; anti-Flt-1 (sc-316), anti-KDR (sc-6251), anti-Kinesin heavy chain (sc-13359), anti-Stanniocalcin (sc-14352), and anti-Grb2 were obtained from Santa Cruz Biotechnology Inc., and anti-β 1 integrin subunit mAbs (4B4), from Coulter Inc.
Affinity-purified polyclonal antibody (Ab) specific for BMP-5, the blocking peptide for the BMP-5 Ab, and the recombinant human BMP-RIA-Fc chimera were purchased from Research Diagnostics (Flanders, NJ).
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