Words similar to affinity-purified

Example sentences for: affinity-purified

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The affinity-purified polyclonal antibodies against threonine558-phospho-moesin were prepared as described previously [ 43].
Finally, the data presented on MCM2 expression by IHC were obtained with affinity-purified polyclonal rabbit antibodies.
Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.
The rabbit antiserum obtained was affinity-purified by passage over a Sulfolink column (Pierce, Rockford, IL, USA) to which disulfide linkage of the peptide had been used to immobilize the peptide.
As shown in Figure 4, affinity-purified mactinin antisera reacted with several 30-40 kDa proteins, including 31 kDa mactinin in each of the samples tested.