Example sentences for: affinity-purified

How can you use “affinity-purified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).

  • Finally, the data presented on MCM2 expression by IHC were obtained with affinity-purified polyclonal rabbit antibodies.

  • Antipeptide polymerase β antibodies were affinity-purified from rabbit serum [ 17 ] .

  • Mouse monoclonal Ab 5B88 to β-galactosidase (GIBCO) was used at a concentration of 2.5 g/ml, followed by rhodamine-conjugated, affinity-purified goat anti-mouse IgG antibody (Boehringer Mannheim, Indianapolis, IN).

  • Affinity-purified anti-α 2 β 1 integrin (MAB1988), anti-α v β 3 integrin (LM609), anti-MMP2 (MAB13405), and anti-VE-cadherin (MAB1989) monoclonal antibodies (mAbs) were purchased from Chemicon Intl; anti-Flt-1 (sc-316), anti-KDR (sc-6251), anti-Kinesin heavy chain (sc-13359), anti-Stanniocalcin (sc-14352), and anti-Grb2 were obtained from Santa Cruz Biotechnology Inc., and anti-β 1 integrin subunit mAbs (4B4), from Coulter Inc.


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