Example sentences for: affinity-purified

How can you use “affinity-purified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The affinity-purified polyclonal antibodies against threonine558-phospho-moesin were prepared as described previously [ 43].

  • Following incubation in blocking buffer (10 mM Tris-HCl, pH 7.5, containing 10% non-fat dry milk, 100 mM NaCl, and 0.1% Tween 20), the membranes were probed with an affinity-purified rabbit polyclonal IgG directed against caspase-8 (Caspase-8 p20, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1:200 dilution in blocking buffer, overnight at 4°C.

  • A band for RIIβ was detected in contrast to a report of no RIIβ detection in soleus or extensor digitorum longus muscles [ 18 ] , which might be explained by the presence of nerve fibers or adipocytes wherein RIIβ is prevalent [ 27 ] . The blot for the affinity-purified D-AKAP1 antibody is shown because the protein-A purified antibody displayed additional, unexplained bands; however, no difference in fluorescence labeling was observed when using antibodies purified either way.

  • The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).

  • Mouse monoclonal Ab 5B88 to β-galactosidase (GIBCO) was used at a concentration of 2.5 g/ml, followed by rhodamine-conjugated, affinity-purified goat anti-mouse IgG antibody (Boehringer Mannheim, Indianapolis, IN).


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