Words similar to affinity-purified

Example sentences for: affinity-purified

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2 -δ protein G-purified guinea pig antibody #1 [ 13], or anti-β 1a affinity-purified sheep #6 antibody [ 44], or with anti-γ 1 affinity-purified guinea pig #16 antibody and the appropriate secondary antibodies [ 11].
The affinity-purified polyclonal antibodies against threonine558-phospho-moesin were prepared as described previously [ 43].
Affinity-purified anti-α 2 β 1 integrin (MAB1988), anti-α v β 3 integrin (LM609), anti-MMP2 (MAB13405), and anti-VE-cadherin (MAB1989) monoclonal antibodies (mAbs) were purchased from Chemicon Intl; anti-Flt-1 (sc-316), anti-KDR (sc-6251), anti-Kinesin heavy chain (sc-13359), anti-Stanniocalcin (sc-14352), and anti-Grb2 were obtained from Santa Cruz Biotechnology Inc., and anti-β 1 integrin subunit mAbs (4B4), from Coulter Inc.
Pip1p and Pcu1p were detected with affinity-purified rabbit antisera.
Following incubation in blocking buffer (10 mM Tris-HCl, pH 7.5, containing 10% non-fat dry milk, 100 mM NaCl, and 0.1% Tween 20), the membranes were probed with an affinity-purified rabbit polyclonal IgG directed against caspase-8 (Caspase-8 p20, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1:200 dilution in blocking buffer, overnight at 4°C.