Words similar to affinity-purified

Example sentences for: affinity-purified

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The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).
Pip1p and Pcu1p were detected with affinity-purified rabbit antisera.
The affinity-purified polyclonal antibodies against threonine558-phospho-moesin were prepared as described previously [ 43].
AP-PH is affinity-purified anti-Boi1 antibody [ 1 ] . AP-101 is affinity-purified anti-Boi1 antibody that was generated in the same manner as AP-PH, except that it came from the serum of a different rabbit.
Affinity-purified recombinant Hepatitis B antigen produced in Saccharomyces cerevisae following transformation with the plasmid pCGA7 was used for detection of antibody responses using an ELISA (Aldevron, Fargo, ND) [ 20 ] . The recombinant antigens were diluted in 0.1 M carbonate buffer (pH 9.6) and coated at 1 ug/100 ul per well in 96-well microtitre plates (Dynex Technologies, INC.