Example sentences for: affinity

How can you use “affinity” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Correct chromosomal integration was confirmed by fluorescence microscopy and immunoblotting with anti-GFP antibodies (rabbit affinity purified IgG; kindly provided by M. Linder, Washington University School of Medicine, St. Louis, MO).

  • Polyclonal antibodies against a N-terminal fragment of MmCOP1 (amino acid 71-270) were generated in rabbits and affinity purified.

  • The two Hh-signaling antagonists, KAAD-cyclopamine and Cur61414, have shown decreased potency on Smo act-expressing cells, leading to the speculation that they may bind this mutant form of Smo less well than the wild-type form [ 11 17 ] . We sought to determine whether the Hh agonist binds Smo actwith a higher affinity, an observation seen with certain ligands and constitutively active mutants of GPCRs [ 29 ] . To perform this experiment, we isolated membranes from cells transfected with a cDNA construct encoding a tryptophan-to-leucine mutation at residue 539 (W539L) of murine Smo.

  • For example, vessels within tumors expressing predominantly the VEGF189 isoform, which has a strong heparin-binding affinity and thus is highly localized, are much less leaky than the vessels in tumors expressing the more diffusible VEGF165 and VEGF121 isoforms [ 45].

  • , unbound) protegrin that remains in the well, and further skew its binding towards higher affinity binding sites on lipid A.


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