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Example sentences for: af

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Multiple tissue Northern blots containing 2 μg poly (A) + RNA (Human Multiple Tissue Northern Blot IV and Human Multiple Tissue Northern Blot; Catalog numbers: 7766-1 and 7760-1; CLONTECH) were hybridized with 32P-labeled human cDNA probes for TOP1(nucleotides 212-1661, GenBank accession number J03250), BTBD1 (nucleotides 84-3196, GenBank accession number AF355402) or BTBD2 (nucleotides 1229-2668, GenBank accession number AF355797) in Rapid-hyb buffers (Amersham Life Science, RPN1635).
The amino acid sequence derived from the nucleotide sequence obtained for the monkey LTBP-3 sequence (GenBank accession number AF395658) shows it to share 98.
EGFP was imaged with an FITC filter set, while ECFP was distinguished from EGFP in co-expression experiments by changing both the excitation and emission filter sets (Exciters: 440AF21 & 500AF25, Dichroic cat# XF 2063, Emitters 480AF & 545AF35; Omega, Brattleboro, VT).
Early splice site choice in mammals involves protein-protein interactions across the exon, as is proposed in the exon definition model of Berget [ 21 ] . Exon definition is mediated in part by SR proteins bridging between the small nuclear ribonucloprotein (snRNP) U1 at a downstream 5' splice site and the U2AF heterodimer at the upstream 3' splice site.
The human genome encodes two proteins with 46% (436 residues, 3% gaps, CAC22147) and 45% (497 residues, 5% gaps, AF353674) identities compared with BTBD2.