Example sentences for: af-

How can you use “af-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Preliminary evidence suggests preferred ligands for PPAR activation are 18 C and 20 C FAs, as FAs shorter than 14 C or longer than 20 C will not fit correctly into the PPAR binding pocket and stabilize the AF-2 helix [ 17 ] . The net effect would be that PPAR co-activators are not recruited to relax nucleosomal DNA and allow transcription machinery to interact with various promoter elements.

  • ERβ Interactions with N-CoR are Dependent on AF-2 and require H12

  • These cofactors interact with nuclear receptors in a ligand-dependent manner and enhance transcriptional activation via histone acetylation/methylation and recruitment of additional cofactors such as CBP/p300 [ 89 ] . CBP/p300 has been implicated in the functions of a large number of regulated transcription factors based primarily on physical interaction and the ability to potentiate transcription when overexpressed [ 90 ] . In the case of nuclear receptors, the interaction with CBP/p300 is ligand-dependent and relies on the conserved nuclear receptor functional domain, AF-2 (activation function 2).

  • In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .

  • Moreover, other mutations in the upper part of the H3-H5 region that comprises the AF-2 surface (D303Y, I310R and K314A on H3; V328R and L331R on H5) abolished ERβ interaction with both cofactors.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast