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Example sentences for: af-
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These cofactors interact with nuclear receptors in a ligand-dependent manner and enhance transcriptional activation via histone acetylation/methylation and recruitment of additional cofactors such as CBP/p300 [ 89 ] . CBP/p300 has been implicated in the functions of a large number of regulated transcription factors based primarily on physical interaction and the ability to potentiate transcription when overexpressed [ 90 ] . In the case of nuclear receptors, the interaction with CBP/p300 is ligand-dependent and relies on the conserved nuclear receptor functional domain, AF-2 (activation function 2).
Moreover, other mutations in the upper part of the H3-H5 region that comprises the AF-2 surface (D303Y, I310R and K314A on H3; V328R and L331R on H5) abolished ERβ interaction with both cofactors.
Thus, ERβ interactions with N-CoR are dependent on the AF-2 surface (including H12) and, in this regard, resemble those of ERβ and GRIP1.
Moreover, this interaction is dependent upon ERβ AF-2, including H12, and is competed by NR box peptides but not ID peptides.
This sequence does not exactly conform to the LXXLL consensus, but contains features (underlined) that resemble the ERβ H12 region (L L LE ML ), and artificial ERβ interacting LXXLL peptides (293, P NLIS LLS ; D47, PL LLS LLS ), both of which bind to the ERβ AF-2 surface [ 43 44 45 46 47 ] . Moreover, the presence of a proline residue amino-terminal to the hydrophobic groups is typical of so-called class II LXXLL motifs which are found in ERβ interacting cofactors such as TRAP220 and RIP140 [ 45 ] . Finally, the unusual C-terminal hydrophobic pair (ML) has been observed in ERα and ERβ H12 [ 43 44 48 ] , and in RIP140 NR boxes [ 19 ] .
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