Example sentences for: af-

How can you use “af-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Moreover, this interaction is dependent upon ERβ AF-2, including H12, and is competed by NR box peptides but not ID peptides.

  • Previously we characterized another RARα mutant in which the receptor protein was truncated after amino acid 403, thereby deleting the AF-2 domain [ 11 ] . This truncated receptor exhibited similar properties to the S77A mutant (growth inhibition and repression of RA responsive gene expression including EGFR).

  • NRs consist of three domains, the N-terminal domain (which contains a context-specific activation function AF-1), the central DNA binding domain (DBD) and the C-terminal ligand binding domain (LBD), which contains a hormone-dependent activation function, AF-2.

  • In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .

  • The LBD utilizes a large hydrophobic cleft composed of residues along H3 and H5 to bind IDs [ 17 ] , and a smaller hydrophobic cleft that is composed of residues in the upper part of H3 and H5 and H12 (and corresponds to AF-2) to bind NR boxes [ 18 ] . Thus, agonists promote coregulator exchange by promoting the packing of H12 over the lower part of the ID binding region, an event that simultaneously completes the coactivator binding surface.


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