Words similar to adp-ribosylation
Example sentences for: adp-ribosylation
How can you use “adp-ribosylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
DGW drafted the manuscript, performed ADP-ribosylation experiments, adhesion and spreading assays, and immunofluorescence.
The phenotype induced by ADP-ribosylation of Rho in T lymphocytes appeared different than that induced in fibroblast models, where microinjection of C3 results in fibroblast retraction of pseudopodia, with the cell body rounding up eventually to become non-adherent [ 17 ] . Video time-lapse microscopy demonstrated the elongation of aberrant pseudopods in HPB-ALL cells plated on the β1 substrate VCAM-1, rather than an altered retraction of already formed pseudopods.
Previous reports have demonstrated that ADP-ribosylation of B-lymphoid cell lines by C3 treatment inhibits phorbol ester induced cellular homotypic aggregation [ 18 ] . Also, phorbol ester and chemoattractant (IL-8 and fMLP) induced pre-B lymphoblastoid adhesion to VCAM-1 and neutrophil adhesion to fibrinogen was abrogated in a dose-dependent manner by C3 exoenzyme [ 36 ] . This may indicate that in cells of hematopoietic lineages, the induction of an increased adhesive state may be susceptible to regulation by Rho proteins.
This small member of the Ras GTPase superfamily has been implicated in fibroblast models of integrin dependent actin stress fiber formation, focal contact assembly [ 13 ] , and gene induction via the serum response element [ 14 ] . Studies of Rho function have been facilitated by the use of specific toxins such as Clostridium botulinum toxin C3 which catalyses the ADP-ribosylation and inactivation of Rho [ 15 16 17 ] . The function of this GTPase and related family members such as Rac and Cdc42 has been extensively studied in the fibroblast model.
In the present study, we demonstrate that ADP-ribosylation of the GTPase Rho in HPB-ALL cells does not result in a decrease in constitutively high adhesion to the extracellular matrix component fibronectin.
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