Words similar to acid

Example sentences for: acid

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The cytotoxicity of domoic acid to neuronal tissue in vitro has been investigated over a wide range of concentrations, namely at 10 μM with primary cultures of cerebellar neurons [ 30 31 ] as well as hippocampal slices [ 33 ] , at 100 μM with cultured prenatal rat hippocampal neurons [ 29 ] and at 20 mM with rat lumbar spinal interneurons [ 28 ] . In order to determine a concentration of domoic acid for our in vitro studies that was toxic to cerebellar granule cells after a short-term exposure in our experimental conditions, we completed a viability study using the WST-1 method.
Rabbit polyclonal antibody against RSV-P protein has been described [ 21 ] . Indirect immunofluorescence and nuclear staining with DAPI was performed essentially as described previously [ 33 ] . A549 cells in monolayer, grown on cover slips, were washed in PBS and fixed in ice-cold 10% trichloracetic acid for 15 min, followed by successive washes in cold 70%, 90% and absolute ethanol for 3 min each.
After blotting, the PVDF membrane was stained with 2% amido black in 7% glacial acetic acid and the protein fronts of all lanes were compared for loading accuracy.
However, the enzyme or enzymes responsible for the formation of retinoic acid in the HL60 cells have not been identified.
All other immunocytochemical analyses were performed after fixation in 4% paraformaldehyde and 0.15% picric acid, followed by permeabilization in 0.3% Triton X100.