Words similar to acaa

Example sentences for: acaa

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The cAMP cell surface receptor Car1 ( carA gene) is responsible for sensing secreted cAMP and activating adenylyl cyclase ( acaA gene), which in turn results in the production and secretion of cAMP in order to relay the cAMP pulses [ 38 ] . Normally both of these genes are expressed at very low levels in growing cells, and their expression is induced during the first few hours of development, including a stimulation of expression by the cAMP pulses themselves [ 39 ] .
The forward and reverse first round PCR primers were KK*ACAA*F1: 5'-GGAGAGTTTGCCTGTTCTATGCC-3' and KK*ACAA*R: 5'-CAAATCAATCTTTGGGC AGG-3', respectively.
The PCR product (5 μl) containing the 3'-UTR (ACAA) insertion/deletion polymorphism was mixed with 5 μl of formamide-based stop buffer, heated to 95°C for 10 min, placed on ice, electrophoresed on a 6% acrylamide gel, and then exposed to film.
Both carA and acaA mRNA were detected at least 2 hours earlier in BS153 cells in comparison to Ax4 cells.
The forward and reverse second round PCR primers were KK*ACAA*F2: 5'-AGTCAGGAATGGCTGCACC-3'and KK*ACAA*R: 5'-CAAATCAATC TTTGGGCAGG-3', respectively; the KK*ACAA*F2 primer was end-radiolabeled with 33P-dATP prior to PCR.