Example sentences for: aca

How can you use “aca” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).

  • For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3'; and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'.

  • 122 (GCC TGA GTA TAA GGT GAC TTA TAC TTG TAA TCT ATC TAA ACG GGG AAC CTC TCT AGT AGA CAA TCC CGT GCT AAA TTG TAG GAC TGC CCG GGT TCT ACA TAA ATG CCT AAC GAC TAT CCC TT) and Gp1Wt3.

  • The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.

  • The mutagensis primers were as follows: for mutC, 5' GCTCGACCT CCA TTC TAA TG T GGG CAG GTG GTT TCA CAG 3'; mutK, 5' CCA TTC TAA TTG GGG C TT TTT GTT TCA CAG GGA GGA AGT 3'; mutE, 5' TTT CAC AGG GA C CTT GTT GAC AGT TCA ACT TCA AAC 3'; Xmut1, 5' CAC AGG GAG GAA GTT CTG TCA TCA ACT TCA AAC AGG 3'; Xmut2, 5' GAG GAA GTT GAC AGT CTA AGA CT A AAC AGG GTT GGC AGT 3'; Xmut3, 5' TGA CAG TTC AAC TTC GTT AGT GGT TGG CAG TGA CAC 3'; Ymut1, 5' TGG CAG TGA CAC AGG TGT ACT CTG CCT GCT TCC CCT 3'; and Ymut2, 5' ACA CAG GCC CAC ACT G AT GCG TTC CCC TTT CCA CCC CTG 3'.


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