Words similar to aca
Example sentences for: aca
How can you use “aca” in a sentence? Here are some example sentences to help you improve your vocabulary:
The mutagensis primers were as follows: for mutC, 5' GCTCGACCT CCA TTC TAA TG T GGG CAG GTG GTT TCA CAG 3'; mutK, 5' CCA TTC TAA TTG GGG C TT TTT GTT TCA CAG GGA GGA AGT 3'; mutE, 5' TTT CAC AGG GA C CTT GTT GAC AGT TCA ACT TCA AAC 3'; Xmut1, 5' CAC AGG GAG GAA GTT CTG TCA TCA ACT TCA AAC AGG 3'; Xmut2, 5' GAG GAA GTT GAC AGT CTA AGA CT A AAC AGG GTT GGC AGT 3'; Xmut3, 5' TGA CAG TTC AAC TTC GTT AGT GGT TGG CAG TGA CAC 3'; Ymut1, 5' TGG CAG TGA CAC AGG TGT ACT CTG CCT GCT TCC CCT 3'; and Ymut2, 5' ACA CAG GCC CAC ACT G AT GCG TTC CCC TTT CCA CCC CTG 3'.
The double stranded oligos used were as follows: for the Ets binding site on the CD5 promoter, 5' CGC GTA CAG GGA GGA AGT TGA CCT CGA 3'; for the oligonucleotides containing the CD5X region, 5' AGG CCT AAG TTG ACA GTT CAA CTT CAA ACA CTC GAG 3'; and for the CD5Y oligo, 5' AGG CCT CAC AGG CCC ACA CTG CCT GCT TCC CTG GAG 3'.
A full length, 1.2 kb IDO cDNA was amplified from IFN-γ stimulated RAW cells and cloned into pGEM T-Easy (Promega), using primers; 5' TAG CGG CCG CGT AGA CAG CAA TGG CAC TC 3' forward, 5' TAA GAT CTT ACA CTA AGG CCA ACT CAG 3' reverse, which contain Not I and Bgl II sites respectively.
The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
Primer sequences were: IL-7 [ 65 ] : IL-7 (5') 5'ACT ACA CCC ACC TCC CGC A3'; IL-7 (3') 5'TCT CAG TAG TCT CTT TAG G3'; SCF [ 65 ] : SCF (5') 5'TCT TCA ACT GCT CCT ATT T3'; SCF (3') 5'ACT GCT ACT GCT GTC ATT C3'; TGFβ1 [ 66 ] : TGFβ1(5') 5'GCG GAC TAC TAT GCT AAA GAG G3'; TGFβ1(3') 5'GTT GTG TTG GTT GTA GAG GGC A3'; β-actin [ 67 ] : β-actin (5') 5'GGG TCA GAA GGA CTC CTA TG3'; β-actin (3') 5'GTA ACA ATG CCA TGT TCA AT3'.
Loading...