Words similar to aca
Example sentences for: aca
How can you use “aca” in a sentence? Here are some example sentences to help you improve your vocabulary:
The primers for rat constitutively expressed ribosomal S12, were sense:5'-ACG TCA ACA CTG CTC TAC A-3' and antisense:5'-CTT TGC CAT AGT CCT TAA C-3' and generate a 303 bp cDNA product as previously described [ 63 ] . Controls for RT-PCR included samples without the enzyme reverse transcriptase and samples without template.
., x 7 ] represents the concentrations of the seven proteins: x 1 = [ACA], x 2 = [PKA], x 3 = [ERK2], x 4 = [REG A], x 5 = [Internal cAMP], x 6 = [External cAMP] and x 7 = [CAR1] and the fourteen different k
Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T
Primer sequences were: IL-7 [ 65 ] : IL-7 (5') 5'ACT ACA CCC ACC TCC CGC A3'; IL-7 (3') 5'TCT CAG TAG TCT CTT TAG G3'; SCF [ 65 ] : SCF (5') 5'TCT TCA ACT GCT CCT ATT T3'; SCF (3') 5'ACT GCT ACT GCT GTC ATT C3'; TGFβ1 [ 66 ] : TGFβ1(5') 5'GCG GAC TAC TAT GCT AAA GAG G3'; TGFβ1(3') 5'GTT GTG TTG GTT GTA GAG GGC A3'; β-actin [ 67 ] : β-actin (5') 5'GGG TCA GAA GGA CTC CTA TG3'; β-actin (3') 5'GTA ACA ATG CCA TGT TCA AT3'.
For MIG2, the oligos were HZ045, 5' TGA CCT CGA GAA CAA ACA AAA TAA AAA TAA AAA AAG AGA CTG TGC GGT ATT TCA CAC CG 3'; and HZ046, 5' TTA GAG GAA AAA TGG TGA GAT AAA AAG GGG CCG TAA AGG AGA TTG TAC TGA GAG TGC AC 3'.
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