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Example sentences for: aca

How can you use “aca” in a sentence? Here are some example sentences to help you improve your vocabulary:

2: CTA G AG GGG AAT TGT TAT CCG CTC ACA ATT CCC CTA TAG TGA GTG GTA TTA ATT TCG CGG GAT CGA (XbaI site in bold)
Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T
Following extraction, we first amplified the picornavirus RNA using primers OL26 (5'-GCA CTT CTG TTT CCC C-3') [ 33 ] and OL27 (5'-CGG ACA CCC AAA GTA G-3') [ 33 ] using the Invitrogen SuperScript One-Step RT-PCR with PLATINUM Taq Kit (Invitrogen Corp., Carlsbad, California).
The double stranded oligos used were as follows: for the Ets binding site on the CD5 promoter, 5' CGC GTA CAG GGA GGA AGT TGA CCT CGA 3'; for the oligonucleotides containing the CD5X region, 5' AGG CCT AAG TTG ACA GTT CAA CTT CAA ACA CTC GAG 3'; and for the CD5Y oligo, 5' AGG CCT CAC AGG CCC ACA CTG CCT GCT TCC CTG GAG 3'.
The primers for rat constitutively expressed ribosomal S12, were sense:5'-ACG TCA ACA CTG CTC TAC A-3' and antisense:5'-CTT TGC CAT AGT CCT TAA C-3' and generate a 303 bp cDNA product as previously described [ 63 ] . Controls for RT-PCR included samples without the enzyme reverse transcriptase and samples without template.