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Example sentences for: ycaf

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This region can, by itself, activate transcription when targeted to the DNA via a heterologous DNA-binding domain [ 5 ] . On the other hand, the LRR plus C-terminal region of yCCR4 cannot mediate transcription activation, but is involved in protein-protein interactions [ 5 ] . The yCCR4 LRR is necessary for the binding of several components of the CCR4-NOT complex, including yPOP2 -and mCAF1- [ 11 ] , yDBF2 [ 2 ] , yCAF4 and yCAF16 [ 8 ] , and deletion of the yCCR4 N-terminus does not impair these associations.
yDBF2, yCAF4 and yCAF16) of the 1.9 MDa CCR4-NOT complex.
The 1.0 MDa complex contains yCCR4, yPOP2 (also referred to as yCAF1) and at least five yNOT1-5 proteins [ 5 6 7 ] . In this complex, yPOP2 independently binds to yCCR4 and yNOT1, and is absolutely required for yCCR4 to associate with the 1.0 MDa complex [ 6 7 ] . In the 1.9 MDa complex, yCCR4 binds to proteins such as yDBF2, a cell-cycle regulated protein kinase [ 2 ] , yCAF4 and yCAF16, and is essential for the interaction of both yCAF4 and yCAF16 with ySRB9, a component of the RNA polII holoenzyme [ 8 ] . yCCR4 was also reported to be associated with Paf1, Cdc73, and Hpr1 in a RNA polII complex distinct from the SRBP-containing holoenzyme [ 9 ] . Accordingly, yCCR4 contains, in its central region, a leucine-rich repeat (LRR) domain [ 10 ] which was demonstrated to be necessary for yCCR4 binding to the yPOP2 [ 5 11 ] , yDBF2 [ 2 ] , yCAF4 and yCAF16 [ 8 ] components of the CCR4-NOT complex.