Words similar to wi
Example sentences for: wi
How can you use “wi” in a sentence? Here are some example sentences to help you improve your vocabulary:
The presence of mouse Dazap1 in the radiation hybrids was determined by PCR and the results were sent to the MIT server www.gonome.wi.mit.edu/mouse_rh/index.hlmlfor computerized physical mapping of the gene.
Poly(A) +RNA was purified from total RNA using PolyTract oligo(dT) columns (Promega, Madison, WI).
Plasmids containing confirmed differentially expressed templates were re-cultured and plasmid DNA was extracted (Wizard Plus Mini-prep DNA Purification System, Promega Corporation, Madison, WI) and subjected to dideoxy chain termination sequencing (Applied Biosystems, Model 373A Automated Sequencer, Oklahoma State University Recombinant DNA/Protein Resource Facility).
Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).
The pRET2.EGFP or pRET2.COX2 plasmids were transfected into ecotropic Phoenix E packaging cells by the calcium phosphate precipitation method, using the ProFection kit (Promega, Madison, WI, USA).
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