Example sentences for: wi

How can you use “wi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Primers were designed with the Primer3 software http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiand were synthesized by Sigma-Genosys.

  • Tissues from Subject #3 and 4, who were 27 and 68 years old, were obtained in the form of slides made from paraffin-embedded sections of testis, epididymis, vas deferens, and seminal vesicles through Novagen (Madison, WI).

  • The PCR mix per reaction included 10 μl SYBR Green buffer, 1.5 mM MgCl 2 , 1 μM dNTP mix (with dUTP), 0.5 μl CMV primer mix, 2.5 units Ampli Taq gold, 0.05 units of uracil-N-glycosylase (UNG, Epicentre, Madison, WI) that had been diluted 1:10 in UNG buffer, and molecular grade water to bring the volume up to 45 μl with the addition of 5 μl of cDNA or standard.

  • Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.

  • Taq polymerase, deoxynucleotide triphosphates and Taq buffer were from Promega (Madison, WI, USA).


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