Words similar to wi

Example sentences for: wi

How can you use “wi” in a sentence? Here are some example sentences to help you improve your vocabulary:

One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
To generate specific probes for Northern blot hybridization experiments, primers based on the rat accession number provided by Affymetrix were designed, primer pairs were chosen using the Primer3 software http://www-genome.wi.mit.edu/from the 1000 most 3' nucleotides within each accession sequence.
Further sequence analysis was carried out with the Genetics Computer Group 10 (GCG; Madison, WI) program.
The animal room was maintained on a 14L:10D cycle, and room temperature was 25-28°C; food (Fox Reproduction Food; Milk Specialties Co., New Holstein, WI) and acidified water (pH= 4.2) were provided ad libitum . Males (n = 6 proven breeders) and females were caged individually and held on different shelving racks, separated by at least 2 m.
24 (CCC GGA ATT CTA TCC AGC TGC ATG; 0.4 μM) in PCR buffer (10 mM Tris-HCl, pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 0.1% Triton X-100, 0.005% gelatin), dNTPS (200 μM) and Taq DNA polymerase (Promega, Madison, WI; 1.5 units).