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Example sentences for: wi

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3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).
Madison, WI).
Ninety-six plasmids for each tester were randomly selected and plasmid DNA was extracted (Wizard SV96 Plasmid DNA Purification System, Promega Corporation, Madison, WI) and eluted into 100 μl sterile nuclease-free water.
The insert of a Dazap1 cDNA clone P21, which encoded the C-terminal portion of DAZAP1 (starting from aa #197), was cloned in-frame into the Eco RI/ Xho I sites of an expression vector pET32b (Novagen, Madison, WI).
A reporter construct containing the basal promoter of the rat RIIβ 5'-flanking region (-394 to -123) upstream of a CAT-reporter gene (pCATbasic; Promega, Madison, WI) was used for assaying RIIβ promoter activity [ 18 ] . Expression vectors for USF1, USF2a and USF2b inserted 3' of the CMV promoter in pCR™3 (Invitrogen, Groningen, The Netherlands) were used to overexpress USF isoforms [ 12 ] . C/EBPβ LAP (full-length) and LIP (DNA-binding domain) expression vectors created in pCMV™3 (Stratagene, La Jolla, CA) were appreciated gifts from Dr. Shizua Akira (Hyogo College of Medicine, Hyogo, Japan).