Example sentences for: wht
How can you use “wht” in a sentence? Here are some example sentences to help you improve your vocabulary:
To examine when Ppt1p is expressed and where it is localized, a yeast strain (WHT4-1) was generated in which the PPT1 gene was replaced with cDNA encoding hemagglutinin-tagged Ppt1p (HA-Ppt1p).
Wild type (W303) cell and WHT4-1 cells were grown in YPAD medium.
Total RNA was prepared from W303 and WHT4-1 yeast cells using hot acid phenol extraction [ 41 ] . For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.
Cells were prepared for immunocytochemistry as described by Harlow and Lane [ 40 ] . Briefly, WHT4-1 cells were grown to early log-phase (OD 600 nm 1.0-1.