Words similar to v-crk
Example sentences for: v-crk
How can you use “v-crk” in a sentence? Here are some example sentences to help you improve your vocabulary:
The JNK pathway is regulated by interaction of c- or v-crk with Cas via the substrate domain [ 38 ] . In determining which pathways are affected in our stable cell lines generated, we determined if JNK was activated in the v-crk transformed cells, and if the tyrosine phosphorylated Cas chimera was affecting this activation.
These data suggest that the kinase inactive fusion with the Cas SD is either transiently tyrosine phosphorylated in cells and v-crk protects them from being dephosphorylated by interacting with them via its SH2 domain, or alternatively, that v-crk induces their tyrosine phosphorylation.
Investigators have shown that v-crk interacts via its SH2 domain with the substrate domain of Cas [ 6 ] . Association of v-crk with endogenous Cas is believed to protect the phosphorylation status of this molecule due to protection from phosphatases [ 12 ] . Expression of an unphosphorylated Cas SD along with v-crk should therefore result in stable tyrosine phosphorylation of the Cas SD if they interact.
As our tyrosine phosphorylated Cas SD chimera can interact with v-crk, we decided to find out if the chimera would affect cell growth and/or transformation in normal cells and those transformed with v-crk.
The v-crk SH2 domain interacts with YXXP motifs upon phosphorylation, suggesting that both of these regions may be able to interact with v-crk [ 37 ] . Fusions of the kinase inactive Src kinase domain and either the full Cas SD, the 5' region of the Cas SD (Cas(5'SD), containing 4 YQXP motifs) or the 3' of the Cas SD (Cas(3'SD), containing 9 YDXP motifs), are not stably tyrosine phosphorylated when expressed alone in cells (data not shown and see Fig.
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