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Appendix V Selected Books and Articles
A significant difference between the Ifk kinase domain and yeast GCN2 kinase domain is the length of the insert sequence between subdomain IV and V, which is around 290 amino acids in the Dictyostelium Ifk protein and 110 amino acids in yeast GCN2.
analyst ABBREVIATIONS biotron bob (4) quadriphony butter pat ADP reticulosis delocalize, v.
Recombinant HA-tagged NFATp proteins (approximately 500 ng) were incubated with calcineurin (500 ng, Sigma) and calmodulin (200 ng, Sigma) in 21 μl of buffer that had the following final concentrations of components: 7 mM Tris-HCl, 5 mM Hepes (pH 7.6), 90 mM NaCl, 15 mM betamercaptoethanol, 0.3 mM DTT, 1 mM EDTA, 30% (v/v) glycerol, 3 mM MgCl 2 , and 1.5 mM MnCl 2 . Control reactions were performed with NFATp proteins in the absence of added calcineurin and calmodulin.
However, we did find significant differences in ThDP uptake capacity with respect to cell type as revealed by variation in the V