Words similar to utp
Example sentences for: utp
How can you use “utp” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reaction buffer contained 10 mM dithiothreitol (DTT), 0.5 μM of ATP, CTP, and GTP, 2.5 μM of UTP, and 5 μl of 800 Ci/mmol [α- 32P] UTP at 10 mCi/ml (Dupont NEN).
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
Digoxygenin sense and antisense RNA probes were generated by labeling with digoxygenin-UTP during transcription.
5 mM) and α- 32P UTP (NEN, Boston, MA; 20 μCi; 3000 mCi/mmol), and incubated at 37°C for 2 hours.
Moreover, analysis of individual transcripts, which were enzymatically digested into mononucleosides, showed equivalent incorporation rates of biotin-11-UTP for both control samples and target samples containing modified ATP.
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