Words similar to utp
Example sentences for: utp
How can you use “utp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Neither apoptotic nor proliferation rates were affected by the status of Pten when measured by the TUNEL (terminal deoxynucleotidyltransferase-mediated UTP end labeling) assay, which labels the ends of fragmented DNA present in apoptotic cells, and immunohistochemical staining for Ki67, a cell proliferation marker (data not shown).
Poly(A) +cDNA was transcribed in vitro by incorporation of biotinylated CTP and UTP (Enzo Diagnostics, Farmingdale, NY) using a BioArray High Yield RNA Transcript Labeling kit according to the manufacturer's instructions.
Reverse transcription was started by adding dNTPs to a final concentration of 1 mM dATP and 500 μM each: dCTP, dGTP, dTTP and 5-(3-aminoallyl)-2'-deoxyuridine-5'-triophosphate, (aa-dUTP) (Sigma), with 150 units of StrataScript (Stratagene, La Jolla, CA).
Substrate transcripts were labeled by incorporation of [ 32P]UTP (10 μCi/ml; Nen Life Science Products, Inc.).
Moreover, analysis of individual transcripts, which were enzymatically digested into mononucleosides, showed equivalent incorporation rates of biotin-11-UTP for both control samples and target samples containing modified ATP.