Words similar to utp

Example sentences for: utp

How can you use “utp” in a sentence? Here are some example sentences to help you improve your vocabulary:

The hCK-5 multiprobe template set was used to synthesize RNA probes for the chemokines lymphotactin, RANTES, IP-10, MIP-1α, MIP-1β, IL-8, MCP-1, and I-309 as well as the house-keeping genes L32 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) labeled with [α- 32P]UTP using T7 RNA polymerase.
Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.
Specific activity of the [ 32P]UTP and the base composition of each substrate molecule were used to calculate substrate concentration.
A very recent report used amino C6dT-modified random hexamers to prime cDNA synthesis in conjunction with aminoallyl-dUTP and increased fluorescence intensity enough such that as little as 1 μg of total RNA from cell lines gave sufficient signal for cDNA microarray hybridization [ 14 ] . The reliability of this method with human tumor specimen warrants further testing.
The transcription reaction mixtures contained lug of linearized DNA; 0.5 mM(each) ATP, CTP, and UTP; 0.1 mM GTP; 0.5 mM cap analog (NEBL); 10 mM DTT; 40 U of Rnasin (Promega); 30 U of SP6 RNA polymerase; and 1 × SP6 RNA polymerase buffer (Promega) in a volume of 30 ul.