Words similar to utp
Example sentences for: utp
How can you use “utp” in a sentence? Here are some example sentences to help you improve your vocabulary:
With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.
It is of interest to note that the results we report here differ from those obtained by others [ 23 ] . Those studies reported increases in signal intensity for targets containing 5-methyl UTP while completely substituted targets containing DAP had the opposite or no effect.
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
Substrate transcripts were labeled by incorporation of [ 32P]UTP (10 μCi/ml; Nen Life Science Products, Inc.).
The biotin-11-UTP peak was measured at an absorbance of 294 nm with the extinction coefficient = 13000 M -1cm -1.
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