Example sentences for: urokinase

How can you use “urokinase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Beads were placed in one of the following solutions: 3.1 mg/ml of recombinant rat PN-1; 2.3 mg/ml of trypsin (type III bovine pancreas, Sigma, St. Louis, MO); 1 mg/ml urokinase plasminogen activator (uPA, Sigma, St Louis, MO); 1 or 10 mg/ml soybean trypsin inhibitor (Sigma, St Louis, MO); 10 mg/ml type IV chick egg white purified ovoinhibitor (Sigma, St Louis, MO) at 4°C overnight and used the next day.

  • The Ad Adapt mhAB encodes for a murinised form of the human ATF-BPTI [ 21 ] . In this construct the mhAB, is preceded by the native secretion signal peptide of the human urokinase.

  • We have shown that α-actinin is abundant in the bone marrow stroma matrix, presumably at focal adhesion sites [ 5 ] . We have also reported that a 31 kDa amino-terminal α-actinin fragment, which we have named mactinin, is generated by the degradation of extracellular α-actinin by monocyte-secreted urokinase [ 6 ] . Furthermore, we have demonstrated that mactinin is present in inflammation caused by Pneumocystis carinii pneumonia, by examining bronchoalveolar lavage fluid from mice with infection [ 6 ] . It was not present in mice not challenged with P. carinii , suggesting that inflammaton is necessary for mactinin formation.

  • After 2 h of incubation at 37 °C, 50 μl detection reagent containing a modified urokinase and S-2444 peptide substrate was added to all wells.

  • In this report, both the active product of urokinase degradation of α-actinin formed in vivo [ 6 ] and the active recombinant actin-binding domain, which are of similar molecular masses, will be referred to as mactinin.


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